叶酸在预防胎源性子宫内膜异位症易感基因HOXA10异常甲基化中的作用

Effect of folic acid in preventing aberrant methylation of fetal endometriosis susceptibility gene HOXA10

目的通过检测和比较子宫内膜异位症（EMS）孕妇有无补充叶酸其女性胎儿脐血EMs易感基因HOXA10启动CpG岛甲基化状态，探讨叶酸优化官内环境的作用。方法收集标本2010年1月,--2012年12月21例患有EMS孕妇服用和15例未服用叶酸的女陛胎儿脐血标本，利用甲基化特异性聚合酶链反应（MsP）及亚硫酸氢盐g-c,后测序（BSP）检测和比较各组HOXA10的甲基化率。结果补充叶酸组女性胎JLEMS易感基因HOXA10甲基化率显著降低，两组差异有统计学意义（P〈0．05）。结论提示叶酸在预防胎源性EMS易感基因HOXA10异常甲基化中可能起一定作用。

Objective To detect aberrant methylation in the promoter region of fetal endometriosis susceptibility gene homeobox-10 （HOXA10） in women with and without folic acid supplementation and explore the effect of folic acid in optimizing intrauterine environment. Methods Thirty-six cord blood specimens were collected between January, 2010 and December, 2012 from pregnant women with endometriosis, including 22 with folic acid treatment and 15 without. Methylation-specific polymerase chain reaction （MSP） and bisulfite salt modified sequencing （BSP） were employed to detect aberrant methylation of HOXA10 gene in these specimens. Results The methylation rate of HOXA10 gene differed significantly between pregnant women with endometriosis taking folic acid and those who did （P〈0.05）. Conclusion Folic acid treatment can significantly reduce the methylation rate of fetal endometriosis susceptibility gene HOXA10.