茶多酚对前列腺癌细胞DU145生长的影响

Tea polyphenols inhibits the proliferation of prostate cancer DU145 cells

目的:探讨茶多酚对前列腺癌细胞生长的影响及其作用机制。方法:选取激素非依赖性前列腺癌细胞系DU145为研究对象,在药物组的培养基中加入茶多酚使其终浓度分别为50、100、250、500μg/ml,对照组加入正常细胞培养基。各组细胞培养48 h后,采用MTT比色法检测细胞生存率,然后通过Western印迹分析和荧光定量RT-PCR法检测各组细胞survivin基因表达情况。结果:加入茶多酚溶液48 h后,各药物组细胞存活率分别为0.97±0.12、0.71±0.07、0.20±0.03和0.08±0.01,与对照组相比,除50μg/ml组(P=0.42)外,其余3个药物组细胞存活率均明显低于对照组(P﹤0.01)。随茶多酚作用时间增加,各组细胞存活率呈现下降趋势,到96 h时,除50μg/ml组,其余3组细胞存活率均在5%以下。加药48 h后,对照组、100、250、500μg/ml药物组的sur-vivin表达条带灰度值分别为15 075±48、13 425±31、2 017±24、1 274±22,与对照组相比,3个药物组survivin蛋白表达均明显减少(P均﹤0.01)。另外,50、100、250、500μg/ml药物组给药48 h时的survivin mRNA量分别为0.74±0.03、0.64±0.02、0.52±0.01、0.21±0.02,均明显少于对照组(P均﹤0.01)。结论:茶多酚可以抑制前列腺癌DU145细胞的生长,且这种作用可能与survivin基因表达减少有关。

Objective： To investigate the effect of tea polyphenols on the proliferation of human prostate cancer cells and its possible mechanism. Methods ： We cultured androgen-independent prostate cancer DU145 cells in the medium with different concentrations （50, 100, 250 and 500 μg/ml） of tea polyphenols, and those in the normal medium as the control. After 48 hours of culture, we detected the survival rate of the cells by MTT assay and determined the expression of survivin by Western blot and quantitative RT- PCR. Results： At48 hours, the survival rates of the prostate cancer DU145 cells were 0.97 ±0. 12, 0.71 ±0.07, 0.20 ±0.03 and 0.08 ± 0.01 in the 50, 100,250 and 500 μg/ml tea polyphenols treatment groups, all significantly reduced as compared with the control group （P 〈 0.01 ） except that of the 50 μg/ml group （P = 0.42）. Furthermore, the survival rate continued to decrease with the prolonging of time, dropping below 5% at 96 hours except in the 50 μg/ml group. The grey values of the survivin expression in the 100, 250 and 500 μg/ml tea polyphenols groups were 13 425 ± 34, 2 017 ± 24 and 1 274 ± 22, respectively, at 48 hours, significantly lower than 15 075 ±48 in the control group （P 〈0.01 ）. Moreover, the content of survivin mRNA at 48 hours was markedly lower in the 50, 100,250 and 500 μg/ml treatment groups （0.74 ± 0.03, 0.64 ± 0.02, 0.52 ± 0.01 and 0.21 ± 0.02） than in the control （P 〈 0.01 ）. Conclusion ： Tea polyphenols can inhibit the proliferation of human prostate cancer DU145 cells, which may be associated with the decreased expression of the survivin gene.