IL-8对卵巢癌细胞生长特性的影响及相关机制的研究

Influence of IL-8 on growth characteristics of ovarian cancer cell and related mechanisms

目的研究内源性IL-8对卵巢癌细胞体外增殖能力、细胞周期、细胞凋亡、成瘤能力的影响及相关机制。方法应用MTT法、流式细胞技术、体外软琼脂集落形成实验、RT-PCR及Western blot技术等对IL-8不同表达状态的卵巢癌A2780细胞和SKOV-3细胞的增殖及成瘤能力进行检测。结果与对照组细胞相比,内源性表达IL-8的各组卵巢癌A2780/ssIL-8细胞的增殖能力及其Cyclin D1、Cyclin B1基因和蛋白的表达水平均增高(P<0.05),体外软琼脂集落形成数增多(P<0.05);而在不同程度上抑制内源性IL-8表达的SKOV-3/asIL-8细胞的体外增殖能力及其Cyclin D1和Cyclin B1基因和蛋白的表达水平均降低(P<0.05),细胞的Caspase-3活性增高,体外软琼脂集落形成数减少(P<0.05);进一步采用特异性信号通路阻断剂阻断内源性高表达IL-8的A2780/ssIL-8细胞,其结果显示体外增殖能力及Cyclin D1和Cyclin B1基因和蛋白的表达水平均降低(P<0.05)。结论 IL-8可增强卵巢癌细胞的增殖和成瘤能力,而抑制IL-8表达或特异性阻断IL-8的信号转导通路则可使卵巢癌细胞的增殖及成瘤能力得以抑制。

To study in vitro effects of endogenous interleukin-8 （IL-8） on proliferation and tumorigenesis of ovarian cancer cells and its mechanism, we employed MTT test, flow cytometry, soft-agar colony formation assay in vitro, RT-PCR test, Western blot assay and so on to detect the proliferation and tumorigenesis in vitro of the ovarian cancer cell lines A2780 and SKOV-3 in different IL-8 expression status. Compared with the control group, the cell proliferation, the gene expression and protein levels of Cyclin Dland Cyclin B1 were improved significantly in several groups of ovarian cancer A2780/ssIL-8 cells expressing endogenous IL-8 （P〈0.05）, and the number of colonies formed by A2780/ssIL-8 cells also increased in vitro in soft agar significantly （P 〈 0.05）. On the other hand, the cell proliferation, Cyclin D 1 and Cyclin B 1 gene expression and protein levels of IL-8-depleted SKOV-3/aslL-8 cells were decreased （P〈 0.05） compared with the control, furthermore, their activity of Caspase-3 was increased （P〈 0.05）, but their number of colonies formed was decreased in vitro in soft agar （P〈 0.05）. Meanwhile, after treatment by specific inhibitors to inhibit the signaling pathways correlated with IL-8 in A2780/ssIL-8 cells, their cell proliferation, Cyclin D1 and Cyclin B1 gene expression and protein levels were decreased as compared with the control. Results indicated that IL-8 can accelerate cell proliferation and promote tumorigenesis of ovarian cancer. And the abilities of proliferation and tumorigenesis can be restrained when endogenous IL-8 was depleted or when IL-8 signaling pathways were inhibited.