摘要
目的探讨CD4+CD25+Foxp3+T(Treg)细胞在子宫内膜癌患者外周血中的表达及其与子宫内膜癌发生、发展的关系。方法采用流式细胞技术检测32例子宫内膜癌患者、30例对照组外周血中Treg细胞的表达,采用ELISA法测定血清中细胞因子TGF-β、IL-10的表达。结果子宫内膜癌患者外周血中Treg细胞的比例显著高于对照组(6.96%±3.82%vs 2.37%±1.33%),其差别有统计学意义;TGF-β、IL-10在子宫内膜癌患者血清中的表达分别为(621.02±139.09)pg/ml和(257.07±37.78)pg/ml,显著高于对照组的(506.88±142.33)pg/ml和(220.58±57.11)pg/ml,其差别有统计学意义。结论子宫内膜癌患者外周血中Treg细胞异常增高可能参与了子宫内膜癌的发生发展。
The CD4＋CD25＋Foxp3＋ regulatory T cells （Tregs） play a vital role in the maintenance of self tolerance and immune homeostasis. This study was designed to measure the expression of CD4＋CD25＋Foxp3＋ regulatory T cells in peripheral blood mononuclear cell （PBMC） of patients with endometrial cancer and investigate the role of Tregs in antitumor regulation. The proportion of CD4＋CD25＋Foxp3＋T cells in PBMC were examined with flow cytometry in 32 patients with endometrial cancer, and compared with that of 30 control samples. The patients with endometrial eancer showed a high proportion of CD4＋CD25＋Foxp3＋ T cells, compared to the control group（6.96%±3.82% vs 2.37%±1.33% ）. The expressions of TGF-β and IL-10 in endometrial group were （621.02±139.09） pg/ml and （257.07±37.78） pg/ml respectively, which was higher than those of control group. In conclusion, the Tregs may play an essential role in tumor immunological abnormality in patients with endometrial cancer.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2013年第7期611-614,共4页
Immunological Journal
