电针对鱼藤酮诱导的帕金森病模型大鼠黑质酪氨酸羟化酶、环氧合酶-2表达的影响

Effect of Electroacupuncture Stimulation of "Fengfu"(GV 16) and "Taichong"(LR 3) on Expression of COX-2 and Tyrosine Hydroxylase in Substantia Nigra in Rats with Parkinson's Di-sease

目的:观察电针对鱼藤酮诱导的帕金森病(PD)模型大鼠黑质酪氨酸羟化酶(TH)、环氧合酶-2(COX-2)表达的影响,探讨电针治疗PD的作用机制。方法:健康雄性SD大鼠40只,随机分为正常组、假手术组、模型组、电针组,每组10只。连续28d给大鼠颈背部皮下注射鱼藤酮制备PD模型。造模成功后电针组大鼠施予电针治疗,取"风府""太冲"穴,连续波,频率2Hz,强度1mA,每日1次,连续治疗14d。采用免疫蛋白印迹法检测黑质区TH、COX-2的表达。结果:与正常组、假手术组比较,模型组大鼠中脑黑质区TH蛋白表达明显减少,COX-2蛋白表达明显增多(P<0.01)。经电针治疗后,电针组大鼠较模型组中脑黑质区TH蛋白表达明显增加,COX-2蛋白表达明显减少(P<0.01)。结论:电针治疗可通过降低炎性反应介质COX-2的表达,减轻炎性反应对PD多巴胺能神经元的损伤,从而发挥对多巴胺能神经元的保护和促进修复作用。

Objective To observe the hydroxylase （TH） and cyclooxygenase-2 （COX-2） disease（PD） rats, so as to explore the mechanism influence of electroacupuncture （EA） therapy on the expression of tyrosine proteins of Substantia Nigra cells（SNc） in the rotenone-induced Parkinson＇s of EA underlying improvement of PD. Methods Forty rats were randomly di- vided into normal, sham-operation （sham）, model and EA groups （n = 10/group）. The PD model was established by successive subcutaneous injection of rotenone （highly selective lesions of nigrostriatal dopaminergic neurons） for 28 days. EA （2 Hz, 1 mA） was applied to bilateral ＂Fengfu＇（GV 16） and ＂Taichong＂（LR 3） for 20 rain, once daily for 14 days. The expression levels of TH and COX-2 proteins in the Substantia Nigra of midbrain were detected with Western blotting. Results Compared with the normal group, the expression level of TH protein in the model group was significantly decreased（P〈0.01）, and that of COX-2 protein in the model group was significantly increased （P〈0.01）. After the EA treatment, the expression level of TH in the EA group was obviously upregulated （P〈0.01）, and that of COX-2 protein in the EA group was considerably down-regulated （P〈0.01）. No significant differences were found between the normal and sham groups in the expression levels of TH and COX-2 proteins （P〉0.05）. Conclusion EA therapy can decrease inflammation mediator COX-2 protein expression and upregulate TH protein expression in the Substantia Nigra of midbrain in PD rats, which may contribute to its effect in relieving PD in clinic.