摘要
采用80%乙醇加热回流和大孔树脂吸附法从山绿茶中提取分离得到总酚酸和总黄酮两个有效部位,分别对两部位中总酚酸和总黄酮及其中主要成分的含量进行了测定,并对山绿茶两个有效部位抗大鼠非酒精性脂肪肝药效作用进行了考察,结果显示,两个有效部位对非酒精性脂肪肝大鼠的肝指数、血脂、转氨酶和肝脏脂肪变性程度均有显著的改善作用。
The flavonoid-rich fraction and phenolic acid-rich fraction were extracted from llex hainanensis Merr. by reflux with 80% ethanol and then isolated by HPD-400 resin, The contents of total flavonoid, total phenolic acid and the main constituents in the two fractions were measured using UV-Vis and HPLC methods. The effects of the two fractions on non-alcoholic fatty liver disease in rats were investigated and the results revealed that both the two fractions showed significant effects in reducing liver index, plasma levels of cholesterol, triglyceride and transaminase, and attenuating steatosis in rats with non-alcoholic fatty liver disease.
出处
《中国野生植物资源》
2013年第3期28-31,46,共5页
Chinese Wild Plant Resources
基金
国家自然科学基金(81073039)
江苏省自然科学基金(BK2011627)
中央高校基本科研业务费专项基金(JKQ2009008)
中央高校基本科研业务费专项基金(JKY2011074)
天然药物活性组分与药效国家重点实验室资助项目(JKGQ201117)
江苏省优势学科资助项目(PAPD)
关键词
山绿茶
总酚酸
总黄酮
非酒精性脂肪肝
Ilex hainanensis Merr.
flavonoid-rich fraction
phenolic acid-rich fraction
non-alcoholicfatty liver disease.
