摘要
本研究通过分析苎麻转录组中的基因注释,首次筛选出8个苎麻WRKY转录因子基因序列。结合拟南芥、水稻、大豆和小麦等作物的10个已报道具有抗旱功能的WRKY转录因子序列进行聚类分析,发现这10个具有抗旱功能的WRKY基因和苎麻的8个WRKY基因的蛋白质编码序列高度保守。利用MEGA软件进一步构建这18个WRKY基因的蛋白质序列进化树,结果发现18个WRKY转录因子可以分为4组,其中Unigene5705与CL3777.Contig1和大豆的Gm-WRKY13同属一组,即WRKY-1类;而Unigene5211 CL5210.Contig1,Unigene14199和拟南芥的AtWRKY2同属WRKY-2类;两个苎麻WRKY基因(Unigene22787和Unigene29108)则和六个已报道的抗旱WRKY基因(AtWRKY57,OsWRKY08,OsWRKY11,OsWRKY72,GmWRKY21和GmWRKY54)同属WRKY-3类;CL34.Contig1,TcWRKY53和ABO3/AtWRKY63同属WRKY-4组。由于所有10个拟南芥、水稻、大豆和小麦WRKY基因具有抗旱功能,因此我们推测苎麻的这8个WRKY转录因子很可能也具有抗旱调控功能。
In this research, we screened 8 ramie WRKY transcription factor genesequences for the first time by analyzing transcriptome gene annotation. 10 WRKY transcription factor sequences related to the function of drought resistance have been reported in arabidopsis, rice, soybean and wheat etc. The 18 gene coding sequences were highly conserved by cluster analysis. We constructed the WRKY gene phylogenetic tree by MEGA software for these 18 WRKY genes, and found that the 18 WRKY transcrip- tion factors could be divided into four groups： Unigene5705, CL3777. Contigl and GmWRKY13 were classified into the WRKY - 1 group; Unigene5211, CL5210. Contigl, Unigene14199 and AtWRKY2 were classified into WRKY - 2 group ; Unigene22787, Unigene29108, AtWRKY57, OsWRKY08, Os-WRKYll, OsWRKY72, GmWRKY21 and GmWRKY54 were classified into WRKY-3 group; CL34. Contigl, TeWRKY53 and ABO3/AtWRKY63 was classified into WRKY-4 group. Since that the function of drought tolerance of 10 WRKY TFs had been characterized, we suggested that these 8 ramie WRKY TFs probably have the potential of drought - tolerance function.
出处
《中国麻业科学》
2013年第3期113-117,共5页
Plant Fiber Sciences in China
