基于微污染水体特征的脱氮功能菌群的构建

Establishment of denitrifying functional bacteria groups based on features of micro-polluted waters

针对微污染水体原位生物脱氮处理中存在的低温、有机碳源含量低及好氧环境问题,采用自适应及菌源生态重组策略构建了生物脱氮功能菌群,并考察了该功能菌群对水库原水的脱氮效果。结果表明,在低温环境条件下对中温脱氮菌种进行液体活化培养,同时通过增加活化培养时间可以提高菌种在低温条件下的脱氮效果,当活化温度为15℃时,各菌株在15,20和30℃环境温度下的TIN去除率基本达到88%以上;采用菌源生态重组策略构建的脱氮功能菌群可在多种极端环境条件下表现出较好的脱氮能力,培养72 h后NO3-和TIN的去除率均可达到90%以上。

Aimed at the issues of low temperature, low content of organic carbon source and aerobic environment existing in the in-situ biological removal of nitrogen for micro-polluted waters, biological denitrifying functional bacteria groups were established by self-adjustment and eco-recombination, and denitrification effects of the functional bacteria groups were studied for reservoir raw water. The results showed that the medium temperature denitrifying strains were cultured activatedly in liquid under low temperature, meanwhile denitrification effects of the strains under low temperature could be improved by increasing the time of activated culture. TIN removal rates could basically be over than 88% at the environmental temperature of 15,20 and 30 ℃ when the activated temperature was 15 ℃. The denitrifying functional bacteria groups established by eco-recombination showed satisfied denitrification ability under many extreme environmental conditions, and the removal rates of NO3- and TIN could be all over than 90% after 72 h of culture.