三参方对慢性萎缩性胃炎大鼠氧自由基及胃黏膜组织形态的影响

Effect of Sanshen Fang on Oxygen Radicals and Gastric Mucous Membrane Tissue in Rats with Chronic Atrophic Gastritis

目的:探讨三参方治疗慢性萎缩性胃炎的作用机制。方法:将实验大鼠随机分为正常组、模型组、维霉素组、三九胃泰组和三参方高、低剂量组,采用复合因素造模法复制大鼠慢性萎缩性胃炎模型,实验8周后,测定各组大鼠血清SOD活性和MDA的含量变化,并观察胃黏膜组织形态变化。结果:与正常组比较,模型组大鼠血清MDA含量明显升高、SOD活性明显降低(P<0.01)。胃黏膜上皮细胞变性、坏死、脱落,腺体有不同程度的萎缩,间质内有大量炎性细胞浸润。与模型组比较,各治疗组大鼠血清MDA含量降低、SOD活性升高(P<0.01或P<0.05),形态学结果显示胃黏膜组织形态有明显改善。结论:三参方能增强SOD活性,降低MDA含量,有效清除氧自由基,具有良好的抗氧化作用;并对胃黏膜的病理形态改变也有较好的改善作用。

Objective: to explore the functional mechanism of Sanshen Fang in treating chronic atrophic gastritis.Methods: The rats were randomly divided into control group,model group,Weimeisu group,Sanjiu Weitai group,high-dose of Sanshen Fang group and low-dose group,models made by composite factors.The experiment lasting for 8 weeks was to detect the changes of serum SOD activity and MDA content,and to observe the gastric mucous membrane tissue change.Results: Compared with control group,MDA content of model group increased significantly,SOD activity decreased significantly(P<0.01);Epithelial cells of gastric mucous membrane had degeneration,necrosis and abscission,glands saw atrophy to different degrees,and there were a great deal of inflammatory cell infiltration.Compared with model group,MDA of treatment groups decreased and SOD increased(P<0.01 or P<0.05);Morphology indicated that gastric mucous membrane tissues were improved greatly.Conclusion: Sanshen Fang can reinforce SOD activity,decrease MDA content,clear oxygen radicals,has the action of antioxidation,and can improve gastric mucous membrane pathological appearance.