芦丁在石墨烯修饰电极上的电化学行为及其灵敏检测

Electrochemical Behavior and Sensitive Detection of Rutin on Graphene Modified Electrode

[目的]研究了芦丁在石墨烯修饰电极上的电化学行为及其测定方法。[方法]采用化学还原法,制备了氨基功能化的石墨烯,并用于构建灵敏的芦丁电化学传感器。采用循环伏安法和差分脉冲法,研究芦丁在该修饰电极上的电化学行为,并用差分脉冲法对芦丁进行检测。[结果]高比表面积和高导电性的石墨烯使芦丁在该传感器上表现出增强的电化学活性。电极反应动力学研究表明,芦丁在该修饰电极表面经历了一个受表面控制的准可逆过程。在最优试验条件下,芦丁的还原峰电流与其浓度在2×10-8～1×10-5 mol/L范围内呈良好的线性关系,检出限(S/N=3)为1.0×10-8mol/L。[结论]该修饰电极具有良好的选择性和稳定性,可实现实际样品中芦丁含量的灵敏检测。

[ Objective ] The aim was to study the electrochemical behavior of rutin on the graphene modified electrode and develop the corr sponding detection method. [ Method] Amino functionalized graphene was prepared with a chemical reduction method and was used to fabri- cate the sensitive rutin electrochemical sensor. Cycle vohammetry and difference pulse method were used to study electrochemical behavior of rutin on modified electrode, and difference pulse vohammetry was adopted to determine rutin. [ Result] Due to the high special surface area and electrical conductivity of graphene, rutin exhibited an enhanced electrochemical activity in the novel sensor. The study of electrode reaction kinetics indicated that rutin undr went a surface-controlled quasi-reversible process. Under the optimized experimental condition, the fabricated sensor displayed excellent analytical performance for rutin detection from 2.0 ×10 ^-8 to 1.0 × 10 ^-5 mol/L with a low detection limit of 1.0× 10^-8 mol/L at 3. [ Conclusion] The proposed electrochemical sensor exhibited good selectivity and stability, and it could be used for real samples.