重金属结合蛋白基因hmbp在转基因拟南芥中的功能分析

Functional Analysis of Heavy Metal Binding Protein Gene hmbp in Transgenic Arabidopsis

[目的]分析重金属结合蛋白基因hmbp在转基因拟南芥中的功能。[方法]利用PCR方法从抗辐射奇异球菌(Deinococcus radiodurans)中克隆hmbp基因,并用沾花法转入拟南芥,用氯化镉处理得到的阳性植株后,检测转基因植株中的丙二醛、脯氨酸、可溶性糖、叶绿素含量、相对电导率及SOD、POD活性。[结果]与野生型相比,镉处理后的转基因拟南芥中脯氨酸、可溶性糖含量及SOD、POD活性增加较多,丙二醛含量增加较少,叶绿素含量减少较少。[结论]研究表明hmbp转基因拟南芥对镉具有很高的抗逆性。

[ Objective ] The paper was to analyze the function of heavy metal binding protein gene hmbp in transgenie Arabidopsis. [ Method ] The hmbp gene was cloned from Deinococcus radiodurans by the PCR method and was transferred into Arabidopsis by the means of floral dip method. The positive plants were obtained after cadmium chloride treatment, and the MDA content, proline content, soluble sugar content, chlorophyll content, relative conductivity, SOD activity, POD activity of transgenie plants were detected. [ Result] Compared with the wild type, the transgenic Arabidopsis had more rise of proline content, soluble sugar content, SOD activity and POD activity of thaliana after CdCl2 treatment, with less rise of MDA content and less abatement of chlorophyll content. [ Conclusion] The study showed that the hmbp transgenic Arabidopsis had high resistance to cadmium.