跑转轮运动与限制性应激对成年小鼠海马神经发生以及空间学习记忆能力的影响

Influence of Wheel Running and Restraint Stress on Hippocampal Neurogenesis and Spatial Learning-Memory in Adult Mice

试图探讨5周跑转轮运动与3周限制性应激对成年小鼠海马神经发生和空间学习记忆能力的影响。Morris水迷宫测试小鼠的空间学习记忆能力。BrdU免疫组织化学法确定新生神经元数量,BrdU+/NeuN+免疫荧光双标记染色法分析BrdU+标记细胞的神经元表型。结果运动组和应激-运动组在定位航行实验中的潜伏期较对照组显著减少(P<0.05),运动组在定位航行实验结束后24 h进行的空间探索实验中目标象限的游泳时间分别较对照组与应激-运动组显著增加(P<0.05)。运动组BrdU+细胞数量分别较对照组与应激组显著升高(P<0.05),并且运动组BrdU+/NeuN+细胞数量分别较对照组与应激组极显著(P<0.01)或显著增加(P<0.05)。运动增加了新生神经元数量,提高了学习记忆能力,应激抑制了运动依赖的海马神经发生,损害了学习记忆能力,运动增强的成年海马神经发生或许是运动提高学习记忆能力的关键机制。

To investigate effects of 5 weeks wheel running and 3 weeks restraint stress on hippocampal neurogene- sis and spatial learning-memory in adult mice. Mice were used to test with the Morris water maze. The number of new neurons was counted by BrdU immunohistochemistry, and the percentage of BrdU ＋ cells eolabeling with NeuN to determine a neuronal phenotype. The latency in the place navigation test of the exercise mice and the stress-ex- ercise mice was significantly reduced （P 〈 0.05 ） than the control group. During the spatial probe test （24 h） af- ter the place navigation test, the time in targeted quadrant with platform of running mice was significantly en- hanced （P 〈 0.05 ）. Compared with the control （P 〈 0.01 ） and the stress （ P 〈 0.05 ） group, only runners bad significantly more BrdU ＋ cells. Meanwhile, BrdU ＋ cells colabefing with NeuN were up-regulated significantly （P 〈 0.05 ）. Therefore, running increases the number of newborn neurons in the hippocampus, and improves the a- bility of spatial learning and memory, stress inhibits neurogenesis as well as impairs spatial learning and memory, which suggested that running-induced adult neurogenesis may be the key regulator of exercise-improved spatial learning and memory.