摘要
[目的]研究卡那霉素（Kan）和潮霉素（Hyg）对甘蓝型油菜、芥菜型油菜和白菜型油菜种子萌发和幼苗生长的影响。[方法]将甘蓝型油菜春K101、芥菜型油菜L0935和白菜型油菜L0926种子分别播种在含有不同浓度Kan或Hyg的1/2MS培养基上,比较不同条件下油菜下胚轴和主根生长的变化。[结果]50mg/L的Kan对油菜的下胚轴生长有促进作用,但对主根的生长有较明显的抑制。Kan对芥菜型油菜种子萌发和幼苗生长的影响最大。Hyg对不同类型油菜的下胚轴和主根生长都有明显的抑制作用。[结论]Kan可用于芥菜型油菜遗传转化实验中转化体的筛选,Hyg对转基因油菜种子筛选的最佳浓度为40~60mg/L。
[Objective] The research aimed to study the seed germination and growth of different rape cultivars, including Brassica napus, Brassica juncea and Brassica rapa, treated with kanamycin or hygromycin at different concentrations. [Method] Brassica napus Chun K101, Brassica napus L0935 and Brassica rapa L0926 seeds were cultured on 1/2 MS medium supplemented with different concentrations of antibiotics. The hypocotyl and taproot changes of different rape cultivated were recorded and analyzed. [Result] It was shown that 50 mg/L kanamycin could stimulate rapeseed hypocotyl growth, but obviously restrain taproot development. Hygromycin apparently inhibited the growth of hypocotyl and taproot of all the three cultivars. [Conclusion] Kanamycin was suitable to screen the transformed seed of Brassica juncea. The optimal concentration of hygromycin to screen transgenic rapeseed was 40-60 mg/L.
基金
Supported by the National High Technology Research and Development Program of China(863Program,2007AA10Z120)
the Doctoral Fund of Henan Polytechnic University(B2011-035)~~
