启动子区甲基化导致ZNF331在结直肠癌中表达缺失

Promoter Methylation-induced Expression Silencing of ZNF331 in Colorectal Cancer

通过分析锌指蛋白331(ZNF331)在结直肠癌中的甲基化及表达情况,探讨ZNF331基因的甲基化与结直肠癌的关系,为结直肠癌的早期诊断和治疗提供新的靶点。采用甲基化特异性PCR(MSP)检测7株结直肠癌细胞系和66例结直肠癌组织标本中ZNF331基因启动子区的甲基化情况。结果7株结直肠癌细胞系中LOVO、RKO、DLD1和HCT116启动子区完全甲基化,SW620和HT29呈部分甲基化,SW480为非甲基化。LOVO、RKO、DLD1、HCT116细胞系中ZNF331mRNA不表达,经5-Aza处理后恢复表达。在SW480、SW620、HT29细胞系中,5-Aza处理前后均表达,处理后表达量增加。在66例结直肠癌组织中,ZNF331启动子区甲基化率为72.7%(48/66),而正常结直肠组织中无甲基化(0/7)。

By analyzing zinc finger protein 331 （ZNF331） methylation and expression in colorectal cancer, to explore the re- lationship of ZNF331 gene methylation and human colorectal cancer and provide a new target for the early diagnosis and treatment of human colorectal cancer. Methylation specific PCR （MSP） was employed to detect the promoter region meth- ylation in 7 colorectal cancer cell lines and 66 cases of primary colorectal cancer. Loss of ZNF331 expression and completely methylation was found in LOVO, RKO, DLD1 and HCT116 cells. ZNF331 expression was restored after 5-aza-2＇-de- oxycytidine treatment. ZNF331 was methylated in 72.7% （48/66） of human primary colorectal cancer. Promoter region methylation was related to tumor differentiation, the ratio of ZNF331 gene promoter region methylation in poorly differen- tiated colorectal cancer was significantly higher than the well--differentiated colorectal cancer （P%0.05）.