基于集成成像的生物微小组织三维信息获取方法

Three-dimensional information acquisition method of biological micro-organism based on integral imaging

提出一种将集成成像和光学显微镜结合实现生物微小组织三维信息获取的新方法。以显微镜为基础,结合集成成像特性,应用光学成像理论设计了与光路匹配的微透镜阵列,研制了微弱光下的三维信息获取系统。与已有方法相比,本文方法采用新的成像光路和三维信息获取器件,因而放大倍数更高,可记录更多的生物微小组织三维信息。针对生物微小组织,利用集成显微成像装置获得了单元像阵列图像实验数据,分析了样本的三维结构和信息,验证了本文系统在微型组织三维信息获取方面的有效性。

A novel method of three-dimensional （3-D） information acquisition of biological micro-organism based on integral imaging and optical microscope is proposed. In this method, a microlens array that matches with optical path is designed and a 3-D information acquisition sYstem under weak illumination is developed using imaging theory and the combination of geometrical optics. Compared with other methods, the magnification is increased by several hundred times, meanwhile more subtle 3-D information of samples is acquired,the eyepiece lens system is retention and the structure of microscope has not been broken,so the aberration can be eliminated more efficiently. Because of whole imaging for samples, the elemental images array can acquire the 3-D information of the sample, not only the surface information but also the internal structure information from the scattered beam by use of the diffuse transmission element, and the information is plentiful. The elemental images arrays are acquired by use of high-sensitivity EMCCD. And then for the biological micro-organism experimental data of element images are acquired by this microscopic integral imaging device, the 3-D structure and information of the sample are analyzed qualitatively and it is efficient for this system to acquire the 3-D information of the biological micro-organism.