DNPH柱前衍生高效液相色谱法测定食品包装材料中的微量甲醛

Determination of Trace Formaldehyde in Food Packaging Materials by DNPH Pre-column Derivation-High Performance Liquid Chromatography

目的建立2,4-二硝基苯肼(DNPH)柱前衍生高效液相色谱法测定食品包装材料中微量甲醛的方法。方法样品浸泡液经DNPH于60℃的水浴中衍生20 min,用环己烷提取,液相色谱仪测定。结果甲醛在0～8.00μg/mL浓度范围内,标准曲线线性关系良好,相关系数r=0.9998,相对标准偏差为0.9%～2.6%,加标回收率为91.0%～101.5%。结论本方法具有灵敏度高、选择性好、精密度和准确度好、简便、快捷等优点。

Objectives To explore the method of DNPH pre-column derivation-high performance liquid chromatography determination of trace formaldehyde in food packaging materials.Methods After immersed into 2,4-dinitrophenyl hydrazine,the sample was derived 20 min in 60℃water bath.Trace formaldehyde in food packaging material was determined by liquid chromatography after cyclohexane extraction.Results The linearity of formaldehyde is in the range of 0-8 μg/mL,correlation coefficient is 0.9998,the relative standard deviation is 0.9%-2.6%,while the recovery rate is 91.0%-101.5%.Conclusions This method is highly sensitive,selective,preciseand accurate,simple and quick which is suitable for determination of formaldehyde in food packaging materials.