拟南芥铁氧还蛋白基因缺失促进花期提前的初步研究(英文)

Loss of Function of Ferredoxin2 Can Promote Flowering in Arabidopsis thaliana

拟南芥的红光/远红光受体光敏色素(PHYs)参与花期调节过程,而铁氧还蛋白色素还原酶(FD-BRs)的一种——植物色素合成酶(HY2)对于光敏色素的合成是必不可少的。研究发现拟南芥铁氧还蛋白——AtFd2的基因缺失突变体(Fd2-KO突变体)在长日照与短日照培养条件下,较其野生型而言均表现出花期提前的表型,而且显示AtFd2与AtHY2在叶绿体中发生互作,并且Fd2突变体对光敏色素的反应受到抑制。推测At-Fd2基因的缺失可能通过影响光敏色素介导的相关生理功能进而对植株的花期进行调节。

In Arabidopsis thaliana, the red and far-red light photoreceptors phytochromes （PHYs） act to involve in regulating flowering. Phytochromobilin synthase （HY2） synthesizes the open chain tetrapyrrole chromophore which is essential to the light-sensing function of phytochromes, and it is a member of the ferredoxin-dependent bilin reductases （FDBRs）. Here, we found a Ds-T-DNA insertion line of Arabidop- sis thaliana for the gene encoding the most major ferredoxin （Fd2, Atlg60950）, which can promote flowering in the process of growth both under long-day and short-day conditions. In this report we show that loss of AtFd2oan promote flowering, AtFd2 interacts with AtHY2 in the chloroplast, and Fd2-KO mutants are impaired in the responses mediated by phytochromes. Together, these results implicate that loss of AtFd2 may promote flowering by impairing the physiology function of phytochromes.