氧浓度对固定化黄孢原毛平革菌合成过氧化物酶的影响

INFLUENCE OF OXYGEN CONCENTRATION ON THE PRODUCTION OF PEROXIDASES BY PHANEROCHAETE CHRYSOSPORIUM

氧浓度对固定化黄孢原毛平革菌 (Phanerochaetechrysosporium)合成过氧化物酶有较大的影响。限碳条件下培养黄孢原毛平革菌 ,生长阶段氧浓度对合成木质素过氧化物酶的影响不明显 ,但在产酶阶段氧浓度的影响却很大。 2 1%氧浓度即空气环境是合成木质素过氧化物酶和锰过氧化物酶适宜的。增高氧浓度 ,胞外蛋白酶活力就会提高 ,不利于过氧化物酶的胞外累积。研究结果表明 ,空气环境下限碳培养黄孢原毛平革菌 ,合成的木质素过氧化物酶最高活力可达 360U/L ,比通纯氧条件下限碳培养时的木质素过氧化物酶要高 160 %。

Oxygen concentration had obvious influences on the production of peroxidases by Phanerochaete chrysosporium . Under limited carbon source culture condition,oxygen concentration in the growth period had little influence on the production of lignin peroxidase,but it had obvious influences in the second metabolism period in which peroxidases were produced.It was found that 21?% O 2(in air)was the optimal oxygen condition on production of peroxidases (including both lignin peroxidase and Mn dependent peroxidase ).Further increase of oxygen concentration depressed the production of peroxidases,because more extracellular protease which was harmful to peroxidases would be produced.At the optimal oxygen concentration (in air 21?% O 2),the maximal lignin peroxidase activity was 360?U/L under limited carbon source culture condition.