Notch-1和Survivin在甲状腺乳头状癌中的表达及意义

Expression and clinical significance of Notch-1 and Survivin in papillary thyroid carcinoma

目的探讨Notch-1、Survivin在甲状腺乳头状癌组织中的表达及临床意义。方法收集80例甲状腺乳头状癌、70例甲状腺腺瘤和50例正常甲状腺组织石蜡标本,采用免疫组织化学SP法检测Notch-1、Survivin蛋白的表达水平。结果在甲状腺乳头状癌、甲状腺腺瘤和正常甲状腺组织中,Notch-1的阳性表达率分别为22.5%、78.6%、88.0%,Survivin的阳性表达率分别为70.0%、12.9%、0%,与甲状腺腺瘤和正常甲状腺组织比较,甲状腺乳头状癌组织Notch-1、Survivin分别呈低表达和高表达状态(P<0.05),且二者表达呈明显负相关(r=-0.512,P<0.01)。Notch-1和Survivin在甲状腺乳头状癌组织的异常表达与肿瘤临床分期、淋巴结转移有关(P<0.05)。结论在甲状腺乳头状癌的侵袭转移过程中存在Notch-1、Survivin的异常表达,对二者的联合检测有助于判断疾病的预后和转归。

Objective To explore the expression and clinical significance of Notch-1 and Survivin in papillary thyroid carcinoma tissues. Methods The expression levels of Notch-1 and Survivin were detected by immunohistochemistry in 80 cases of papillary thyroid carcinoma, 70 cases of thyroid adenoma and 50 cases of normal thyroid gland tissues. The correlation between the expression levels and clinical pathological parameters was analyzed. Results The positive expression rate of Notch-1 in papillary thyroid carcinoma, thyroid adenoma and normal thyroid gland tissues was 22.5% ,78.6% ,88.0%, respectively, and the positive expression rate of Survivin was 70.0%, 12.9% ,0% , respectively, in papillary thyroid carcinoma, thyroid adenoma and normal thyroid gland tissues. As compared with that in thyroid adenoma and normal thyroid gland tissues, Notch-1 in papillary thyroid carcinoma was low-expressed, however, Survivin was over-expressed （ P 〈 0.05 ）, furthermore the expression of Notch-1 was closely correlated to that of Survivin （ P 〈0.01 ）. The abnormal expression of Notch-1 and Survivin in papillary thyroid carcinoma was related to clinical stage and lymph node metastasis of the tumor （ P 〈 0.05 ）. Conclusion The abnormal expressions of Notch-1 and Survivin exist during invasion and metastasis of papillary thyroid carcinoma, and combined detection of the two markers is helpful to evaluating the prognosis and outcome of papillary thyroid carcinoma.