甘草酸二胺对腹膜透析大鼠腹膜纤维化的影响

Effect of diammonium glycyrrhizinate on peritoneal fibrosis in rats receiving peritoneal dialysis

目的探讨甘草酸二铵对腹膜透析大鼠腹膜纤维化的影响。方法将清洁级雄性SD大鼠随机分成正常对照组,不给采取干预措施;生理盐水组,每日腹腔注射0.9%氯化钠注射液20ml;模型组,每日腹腔注射4.25%腹膜透析液20ml,同时腹腔内注射脂多糖(lipopolysaccharide,LPS)0.6mg/kg,3次/周;已酮可可碱治疗组,除与模型组相同处理外,每日加己酮可可碱6mg/kg腹腔注射;甘草酸二胺治疗组,除与模型组相同处理外,每日加甘草酸二铵10mg/kg,腹腔注射。连续28d,于第30天行2h腹膜平衡实验测定腹膜功能,并留取壁层腹膜用光镜观察病理形态。结果正常对照组与生理盐水组腹膜功能及腹膜结构无明显变化,模型组腹膜功能及结构受损最重(P<0.05),甘草酸二胺组及已酮可可碱治疗组腹膜功能及结构损伤较轻(P<0.05),均可明显保护腹膜,2组差异无统计学意义。结论 4.25%腹透液会使大鼠腹膜纤维化,甘草酸二胺可以减轻腹膜的纤维化,减少腹膜结构和功能的恶化。

Objective To investigate the effect of diammonium glycyrrhizinate on peritoneal fibrosis in rats receiving peritoneal dialysis. Methods The male SD rats （ clean grade） were randomly divided into 5 group, the rats in normal control group were not given any intervention ; the rats in NS group were given 0. 9% sodium chloride injection 20ml by intraperitoneal injection daily; the rats in model group were given 4.25% peritoneal dialysis solution 20ml daily, meanwhile, were given lipopolysaccharide （LPS） 0. 6mg/kg, 3 times a week by intraperitoneal injection; the rats in pentoxifylline treatment group, on the basis of model group, were given pentoxifylline 6mg/kg daily by intraperitoneal injection;the rats in diammonium glycyrrhizinate treatment group, on the basis of model group, were given diammonium glyeyrrhizinate lOmg/kg daily by intraperitoneal injection. The trial was performed for continuous 28 days, at the 30th day, 2h peritoneal equilibration test was adopted to determine the rat＇s peritoneum function, and the partial peritoneum tissues of rats were collected and the changes of pathological morphons were observed under light microscope. The experimental data were analyzed with SPSS 13.0. Results There were no significant changes in peritoneum function and structure in normal control group and NS group,however, which in model group were severely damaged （P 〈 0. 05）, and the injury degrees of peritoneum function and structure in diammonium glycyrrhizinate treatment group and pentoxifylline treatment group were comparatively slighter （ P 〈 0. 05 ） , and there were no significant differences in the protective effects on peritoneum between the two groups （ P 〉 0.05 ）. Conclusion The 4.25% peritoneal dialysis solution can cause peritoneal fibrosis in rats, however, diammonium glycyrrhizinate can improve peritoneal fibrosis and relieve the aggravation of peritoneum structure and function.