酸损伤对兔食管下括约肌形态及功能的影响

Effect of acid perfusion on the function and morphology of in lower esophageal sphincter (LES) cells of rabbits

目的 观察酸灌注对兔食管下括约肌 (L ES)形态及功能的影响 .方法 采用 0 .1mol·L- 1 HCL (1m L· min- 130 min· d- 1 for 4d )连续行兔食管灌注 ,测定 L ES的压力变化 .以 Fluo- 3作为钙指示剂 ,用激光共聚焦显微镜测定酸灌注前后细胞内游离钙的浓度变化 ,以及其对乙酰胆碱作用(Ach)的不同反应 .结果 酸灌注后 L ES产生明显的炎症改变 ,L ES压力降低 ,L ES平滑肌细胞内游离钙的浓度 [Ca2 + ]i明显降低 (P<0 .0 1) ,在应用 ACh时正常对照组 L ES平滑肌 [Ca2 + ]i 明显升高 (P<0 .0 1) ,而酸灌注组则无明显变化(P>0 .0 5 ) .结论 酸灌注可使兔 L ES产生明显的炎症改变 ,其平滑肌 [Ca2 + ]i 明显降低 ,可能影响兔 L

AIM To observe the effect of acid perfusion on the morphology and function of rabbit LES. METHODS By using 30 min perfusion of 0.1 mol·L -1 HCl at 5 cm above theLES on 4 consecutive days, we established the model to determine the LES pressure. Intracellular calcium concentration was measured with the fluorescent Ca 2+ indicator Fluo 3 and laser scanning confocal microscopy. RESULTS Significant inflammatory changes appeared after the acid perfusion. LES pressure dropped and [Ca 2+ ] i decreased after the acid perfusion ( P <0.01 ). [Ca 2+ ] i increased both in normal and control subjects ( P <0.01 ) but not in the perfused ones. CONCLUSION Acid perfusion can bring about obvious inflammatory changes in LES such as a significant drop in LES pressure, and reduce the [Ca 2+ ] i in rabbit LES cells, as well as affect the release of intracellular stored Ca 2+ .