实验性瞬时高血压诱发大鼠脑内Fos表达

Expression of Fos-like protein in the rat brain following phenylephrine-induced hypertension

目的 观察大鼠中枢神经系统调节血压的部位 .方法 用苯肾上腺素静脉注射制作大鼠高血压模型 ,以抗 Fos蛋白免疫组织化学方法 ,观察全脑 Fos阳性神经元分布 .结果 高血压诱发下列区域 Fos阳性细胞集中分布 :延髓内脏带、臂旁外侧核、室旁核、视上核 ,杏仁核、终纹床核、额皮质 1,2区、扣带皮质及梨状前皮质 .位于延髓内脏带孤束核内的 A2细胞群和腹外侧区的 A1细胞群分别有 37.6 3%和 5 4.2 5 %的酪氨酸羟化酶阳性神经元表达 Fos蛋白 .结论 本研究表明 ,血压升高引起中枢神经系统内从皮质至延髓广泛的 Fos表达 ,它们互相联系形成一复杂的中枢调控网络 .延髓内脏带内儿茶酚胺能神经元也参与这一调控 .

AIM To investigate the brain areas involved in blood pressure modulation. METHODS Animal models of hypertention were induced by injecting phenylephrine into the vein. Then the distribution of Fos like immunoreactive neurons in the brain was studied immunohistochemically. RESULTS The transient hypertention induced a significant increase in Fos immunoreactivity in the medullary visceral zone, lateralparabrachial nucleus, the paraventricular nucleus, supraoptic nucleus. the central nucleus of amygdala, bed nucleus of stria terminalis, frontal cortex, cingulate gyrus and prepiriform cortex. Approximately 37.63% and 54.25% of tyrosine hydroxylase positive cells were also Fos like immunoreactive in the A2 cell group in the nucleus tractus solitarii and A1 area in the ventralateral medulla. CONCLUSION The present study demonstrates that several brain areas from frontal cortex to medulla were involved in blood pressure modulation. They were connected with each other and formed a complicated regulating network. Catecholaminergic neurons in the MVZ also participate in this regulation.