高效毛细管电泳及其在蛋白质、多肽分析中的应用

High Performance Capillary Electrophoresis and Its Application in Analysis of Protein and Peptide

高效毛细管电泳 (HPCE)是一种分离效率高、检测灵敏度高、样品用量少的分析技术。本文简述HPCE的研究进展及基本原理 ,着重介绍了它在蛋白质及多肽的分离、纯度鉴定、性质研究、结构分析、临床检测、药代动力学研究等方面的应用。

High performance capillary electrophoresis(HPCE) is characterized as an analysis method, which showed high selectivity and high sensitivity, but needed only little sample.In this article,the development of HPCE and its foundamental principle were briefly introduced,and its applications to separation, purity determination,characterization study,structural analysis,clinical monitoring and pharmacokinetics of protein and peptide were emphasized.