用UDPE技术检测感染鼠疫菌植物

Applying UDPE technique to detect infected plants with Y.Pestis

用ＵＤＧ（尿嘧啶糖化酶）防ＰＣＲ产物污染，以两个不同基因为靶序列检测同一病原菌和微孔板杂交－酶联显色检测 ＦＣＲ产物的ＵＤＰＥ（ＵＤＧ－Ｄａｐｌｅｘ ＰＣＲ－ＥＩＡ）技术，检测感染鼠疫菌植物５０份，检出阳性３５份，而常规直接培养阳性仅５份。表明ＵＤＰＥ技术灵敏度高、特异性强。

UDG(Uracil Deoxynucleotide Glycosidase) was used for anti- carryover PCR products.Two different of gents as target sequence were for amplification.And UDPE(UDG-Duplex PCR-EIA) in PCR products were detected by microplate hybrization and enzyme immunoassay(EIA) .50 samples of infected plants with Y.pestis were detected. 35 samples were positive. This method is high sensitive and specific.