2,4-D、NAA对甘蔗胚性愈伤组织形成及分化的影响

Effects of 2,4-D and NAA on Embryogenic Callus Formation and Shoot Regeneration of Sugercane (Saccharum spp.)

以新台糖22(ROC22)、福农94-0403、云蔗03-258及P44为材料,MS为基本培养基,研究切割厚薄不同的外植体及2,4-D浓度对甘蔗愈伤组织诱导的影响,R1、R2及R3三种不同培养基对分化不定芽的影响。结果表明:①外植体切片0.1cm时的诱导率较切段0.5cm高;②切片时最适2,4-D浓度为1～2mg/L,而切段时则以3mg/L时诱导率达最大;③R2和R3分化效果优于R1,R3分化培养基:MS+6-BA 1mg/L+NAA 1mg/L对愈伤组织分化不定芽的效果最佳。

Abstract： Explants of sugarcane varieties ROC22, Funong 94-0403, Yunzhe 03-358 and P44 were cultured on MS medium with various supplements. Experiments were executed to evaluate the effect of different thickness of explants and different concentration of 2,4-D on callus induction as well as three different medium of R1, R2, R3 on shoot regeneration. The results were as the following： firstly, the induction rate of explants of 0.1 cm thick discs was higher than that of 0.5 cm thick segments; secondly, the appropriate 2,4-D concentration for callus induction were 1-2 mg/L for explants of 0.1 cm thick and 3 mg/L for explants of 0.5 cm thick; thirdly,shoot regeneration rating Of the R2 and R3 were better than that of the R1, and the R3 medium： MS supplemented with NAA at 1 mg/L and 6-BA at 1 mg/L was the best medium for shoot regeneration.