不同温度大鼠无血预充深低温停循环模型的建立

Establishment of a temperature-controlled deep hypothermic circulatory arrest model without blood priming in rats

目的建立温度可控的大鼠深低温停循环(DHCA)完全脑缺血模型。方法 23只SD大鼠分为五组。A组:DH-CA 15～20℃(n=5);B组:DHCA 20～25℃(n=5);C组:DHCA 25～30℃(n=5);D组:常温体外循环组(n=5);E组:假手术组(n=3)。七氟烷诱导麻醉,颈静脉置入自制多孔静脉引流管为静脉引流端,左侧股动脉22G套管针穿刺监测血压,鼠尾动脉20G套管针穿刺为动脉灌注端,转机过程中分别于6个时间点抽取血气,记录各时间点血气值及复跳时间。结果 2只大鼠死亡,其余21只大鼠均顺利完成实验过程,DHCA各组心脏复跳后,乳酸值明显升高,血压、心率较D、E组降低。结论本实验模型可用于研究温度及其他干预条件下DHCA对大脑及其他脏器的影响。

Objective To establish a temperature - controlled deep hypothermic circulatory arrest（DHCA） model in rats with complete ischemic brain. Methods Twenty three rats were divided into 5 groups, A： DHCA group 15℃ -20℃ （n = 5 ） ;B： DHCA group 20℃ -25℃ （n=5）; C： DHCA group 25℃ -30℃ （n=5）; D： normthemic CPB group （n=5）; E： sham group （n=3）. After sevoflurane inhalation anesthesia, homemade multiorificed catheter was cannulated in right jugular vein for venous drainage. 22G catheter was cannulated in left femoral artery for monitoring arterial blood pressure, 20G catheter was cannulated in tail artery for the arterial inflow. Blood gas, homodynamic parameters, interval of cardiac recorvery were measured in six time points before the end of the process. Results Twenty one rats survived during the experiment, while two died. After heart resuscitation, comparing to D, E group, lactate of rats in three DHCA groups significantly increased, heart rate and blood pressure declined. Conclusion The model could be used to study pathophysiology of brain and other organs caused by DHCA in different temperature and other interventions.