高强度聚焦超声体外消融犬肾交感神经

Ablation canine renal sympathetic nerve with high intensity focused ultrasound

目的观察不同声功率的HIFU体外消融犬肾交感神经的损伤情况。方法将12只健康杂交犬随机平均分为4组,分别为消融声功率150W组、200W组、250 W组及对照组,以两侧肾动脉为治疗靶区进行连续直线扫描,线长10mm,扫描线的中点与肾动脉重叠,扫描速度6mm/s,重复消融2次。6天后将实验犬处死,对消融后的肾动脉及周围组织进行HE染色及免疫组化染色,观察肾交感神经及肾动脉的变化。结果经不同声功率HIFU消融后,各消融组神经均出现不同程度损伤,而肾动脉血管结构完整,与对照组相比无差异。结论 HIFU能安全且有效地消融犬肾交感神经;声功率越高,肾交感神经损伤越明显。

Objective To investigate the changes of canine renal sympathetic nerve after HIFU ablation with different sound power. Methods Twelve healthy hybridization canines were randomly divided into 4 groups according to sound pow er： 150 Wgroup, 200 W group, 250 Wgroupand control group （eachn：3）. The target area for ablation was bilateralre nal artery, which underwent continuous linear scanning. The line length was 10 ram, scanning the midpoint and renal artery overlap witb speed of 6 mm/s, and ablation was repeated 2 times. Six days later, all dogs were sacrificed, the ablated renal artery and surrounding tissues were harvested for HE staining and immunohistochemical examination, and the changes of renal sympathetic nerve and renal artery were observed. Results After different sound power HIFU ablation, renal sympathetic nerve showed varying degrees of injury in each ablation group, but renal artery vascular structure were still integrity, without obvious anomaly compared with control group. Conclusion HIFU is a safe and effective ablation method of canine renal sympathetic nerve. Higher sound power brought more obvious renal sympathetic nerve damage.