摘要
目的观察神经干细胞(NSC)联合自组装IKVAV纳米纤维凝胶移植促进大鼠脊髓损伤的修复。方法SD大鼠230只,随机分为凝胶治疗组、NSC治疗组、NSC联合自组装IKVAV纳米纤维凝胶移植治疗组、生理盐水治疗组和假手术组。分别于术后第1、3、5、7、14、28、56、92天进行BBB运动功能评分、免疫荧光及免疫印迹观察各组脊髓的修复情况。结果不同治疗方式其BBB评分结果差异有统计学意义(P〈0.01)。联合治疗组第3天BBB评分为(1.6±0.6)分,优于其他各治疗组,且差异有统计学意义(P〈0.001)。联合移植组大鼠损伤脊髓GFAP表达低于其余各组(除假手术组),NF-200表达均高于其他治疗组(除假手术组)。联合移植组BDNF和NGF的表达较其他治疗明显,差异有统计学意义(P〈0.01)。结论IKVAV纳米纤维凝胶、NSC及NSC联合自组装IKVAV纳米纤维凝胶移植均有促进损伤脊髓的功能恢复,NSC联合自组装IKVAV纳米纤维凝胶移植更有促进损伤脊髓功能的恢复的作用。
Objective To observe the effects of neural stem cells (NSC) plus self-assembly isoleucine-lysine-valine-alanine-valine (IKVAV) nanofiber gel transplantation on the promotion of function recovery of spinal cord injury (SCI) in rats. Methods A total of 230 SD rats were randomized into gel, NSC, NSC plus self-assembly IKVAV nanofiber gel transplantation, normal saline and sham-operation groups. Function repair was evaluated by bundle branch block (BBB) score, immunofluorescenee and Western blot respectively at Day 1, 3, 5, 7, 14, 28, 56 and 92 post-operation. Results There were statistically significant differences among bundle branch block ( BBB ) scores of different treatment groups (P〈0. 01 ). Moreover, statistical significance existed between each treatment group and combined transplantation group (P = 0. 000 ). The expression of glial fibrillary acidic protein in combined transplantation group ( rats with spinal injury) was lower than that in other treatment groups ( except for sham operation) and the expression of NF-200 in this group was higher than that in other treatment groups ( except for sham operation). Significant differences existed in the expressions of brain-derived neurotrophic factor and nerve growth factor between combined transplantation and other treatment groups ( P 〈 0. 01 ). Conclusion Transplantation with IKVAV nanofiber gel, NSC and NSC plus self-assembly IKVAV nanofiber gel may promote the repair of SCI in rats. But the method of NSC plus self-assembly IKVAV nanofiber gel is more effective.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2013年第21期1669-1673,共5页
National Medical Journal of China
基金
新疆维吾尔自治区自然科学基金(2011211A059)