神经干细胞联合体外自组装IKVAV纳米纤维凝胶移植治疗大鼠脊髓损伤

Combined application of neural stem cell and self-assembly isoleucine-lysine-valine-alanine-valine nanofiber gel transplantation in the promotion of function recovery of spinal cord injury in rats

目的观察神经干细胞（NSC）联合自组装IKVAV纳米纤维凝胶移植促进大鼠脊髓损伤的修复。方法SD大鼠230只，随机分为凝胶治疗组、NSC治疗组、NSC联合自组装IKVAV纳米纤维凝胶移植治疗组、生理盐水治疗组和假手术组。分别于术后第1、3、5、7、14、28、56、92天进行BBB运动功能评分、免疫荧光及免疫印迹观察各组脊髓的修复情况。结果不同治疗方式其BBB评分结果差异有统计学意义（P〈0．01）。联合治疗组第3天BBB评分为（1．6±0．6）分，优于其他各治疗组，且差异有统计学意义（P〈0．001）。联合移植组大鼠损伤脊髓GFAP表达低于其余各组（除假手术组），NF-200表达均高于其他治疗组（除假手术组）。联合移植组BDNF和NGF的表达较其他治疗明显，差异有统计学意义（P〈0．01）。结论IKVAV纳米纤维凝胶、NSC及NSC联合自组装IKVAV纳米纤维凝胶移植均有促进损伤脊髓的功能恢复，NSC联合自组装IKVAV纳米纤维凝胶移植更有促进损伤脊髓功能的恢复的作用。

Objective To observe the effects of neural stem cells （NSC） plus self-assembly isoleucine-lysine-valine-alanine-valine （IKVAV） nanofiber gel transplantation on the promotion of function recovery of spinal cord injury （SCI） in rats. Methods A total of 230 SD rats were randomized into gel, NSC, NSC plus self-assembly IKVAV nanofiber gel transplantation, normal saline and sham-operation groups. Function repair was evaluated by bundle branch block （BBB） score, immunofluorescenee and Western blot respectively at Day 1, 3, 5, 7, 14, 28, 56 and 92 post-operation. Results There were statistically significant differences among bundle branch block （ BBB ） scores of different treatment groups （P〈0. 01 ）. Moreover, statistical significance existed between each treatment group and combined transplantation group （P = 0. 000 ）. The expression of glial fibrillary acidic protein in combined transplantation group （ rats with spinal injury） was lower than that in other treatment groups （ except for sham operation） and the expression of NF-200 in this group was higher than that in other treatment groups （ except for sham operation）. Significant differences existed in the expressions of brain-derived neurotrophic factor and nerve growth factor between combined transplantation and other treatment groups （ P 〈 0. 01 ）. Conclusion Transplantation with IKVAV nanofiber gel, NSC and NSC plus self-assembly IKVAV nanofiber gel may promote the repair of SCI in rats. But the method of NSC plus self-assembly IKVAV nanofiber gel is more effective.