摘要
目的 研究脑血栓和 (或 )脑腔隙性梗死 (脑腔梗 )患者中凝血因子Ⅻ (FⅫ )对纤溶的影响。方法 用ELISA方法检测脑血栓患者血浆FⅫ∶Ag、FⅫa、βFⅫa水平 ,FⅫ∶C和其他纤溶活性 ;利用MOEA技术筛选是否存在已知常见的FⅫ基因点突变。结果 10 7例脑血栓患者中 ,有 2 2例呈FⅫ∶C下降 ,类似FⅫCRM+表现 ,PLG∶A、α2 AP∶A升高和D 二聚体 (D D)水平降低的同时 ,FⅫa和βFⅫa水平明显降低 ;2 2例FⅫ∶C下降患者均未见已知的FⅫ基因突变。结论 FⅫ∶C下降可能通过降低纤溶酶原的活性 ,在脑血栓形成中发挥重要作用 ;类似FⅫCRM+的FⅫ异常 ,其基因突变与已知基因突变不同 ;FⅫ 334精氨酸、35 3精氨酸所在区域相应的基因点突变可能对FⅫa、βFⅫa水平和纤溶活性下降更重要。
Objective To study the effects of FⅫ on fibrinolysis in patients with cerebral thrombosis. Methods Plasma level of FⅫ∶C, FⅫ∶Ag, FⅫa and β FⅫa and fibrinolysis activities were examined by ELISA. Screening of FⅫ gene mutation by MOEA. Results FⅫ∶C in 22 of 107 patients with cerebral thrombosis decreased, which was similar to the feature of FⅫ cross reacting material positive (FⅫ CRM +). There were significant increase in plasma levels of PLG∶A and α 2AP∶A and decrease in D dimer, moreover, plasma levels of FⅫa and βFⅫa were lower in patients than in controls. FⅫ gene mutation was not found in 22 of 107 patients. Conclusion Decrease of FⅫ∶C may play an important role in cerebral thrombosis by reducing activation of plasminogen. The gene mutation of FⅫ CRM + like abnormal FⅫ was different from the known FⅫ gene mutation. Mutations in the regions of FⅫ Arg (334) and Arg(353) may be more important for the reducing of FⅫa、β FⅫa levels and fibrinolysis activities. FⅫ assay should be included in thrombotic disorder screening.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2000年第9期466-468,共3页
Chinese Journal of Hematology
关键词
脑血栓形成
凝血因子Ⅻ
纤维溶性
基因突变
Blood coagulation factor Ⅻ
Thrombosis
Fibrinolysis
Gene mutation