大鼠皮肤创伤修复过程中转化生长因子β1和β3的表达

Transforming growth factor beta 1 and beta 3 expression during skin wound healing of rats

背景:转化生长因子β在组织创伤修复中发挥核心和关键作用。目的:观察转化生长因子β1和转化生长因子β3在大鼠皮肤瘢痕性创伤愈合过程中表达量及表达部位的变化。方法:制备大鼠皮肤全层切伤模型,长度1.5-2.0cm,深及筋膜层。于伤后0h,12h,1d,2d,3d,4d,5d,6d,7d处死大鼠,取损伤部位皮肤,采用免疫组织化学染色检测各时间点转化生长因子β1和转化生长因子β3的表达,并进行定量分析。结果与结论:免疫组织化学染色显示,在创伤愈合的早期阶段(伤后1-5d),转化生长因子β1和转化生长因子β3免疫阳性颗粒主要出现在上皮细胞、上皮基底层细胞胞浆、巨噬细胞等免疫细胞胞浆及肉芽组织中;随着创伤修复时间的持续,免疫阳性颗粒主要出现在真皮层的成纤维细胞及细胞外基质中。其中转化生长因子β1的表达在创伤后1-5d最强,而转化生长因子β3在创伤后六七天时开始明显表达。可见在大鼠皮肤瘢痕性创伤愈合过程中,转化生长因子β1的表达先于转化生长因子β3,提示转化生长因子β1与胶原形成及创伤修复关系密切,而转化生长因子β3在愈合后期表达量有升高趋势,其可能与创伤后期的组织改建密切相关。

BACKGROUND： Transforming growth factor β plays a key role in the repair of tissue injury. OBJECTIVE： To investigate the expression level and distribution of transforming growth factor β1 and transforming growth factor β3 during healing of skin scarred wound. METHODS： The ful-thickness incised wound model was established in rats, 1.5-2.0 cm long to the fascia layer. Immunohistochemistry was used to quantitatively analyze the expression level and position of transforming growth factor β1 and transforming growth factor β3 in rats at 0, and 12 hours, 1, 2, 3, 4, 5, 6, and 7 days post injury. RESULTS AND CONCLUSION： Results showed that the transforming growth factor β1 and transforming growth factor β3 positive particles mainly distributed in cytoplasm of epithelial cel s and epithelial basilar membrane, macrophage and granulation tissue in the early stage of wound healing （1-5 days post injury）. With prolonged time, the positive particles were mainly found in fibroblast and extracel ular matrix. The expression level of transforming growth factor β1 was up-regulated intensively during 1-5 days after injury, and transforming growth factor β3 was significantly expressed since 6 and 7 days post injury. That is, transforming growth factor β1 was earlier expressed than transforming growth factor β3 during wound healing. Results showed a close relationship between transforming growth factor β1 and col agen formation, as wel as wound repair. The expression level of transforming growth factor β3 was increased in the later stage of wound healing, indicating that transforming growth factor β3 may be related to the remodeling of the wound.