构建组织工程化半月板:细胞、支架及生物因子

Construction of tissue-engineered meniscus: Cells, stents and biological factors

背景:自体或异体移植修复损伤的半月板治疗效果并不理想,应用组织工程化技术重建半月板的研究成为目前的研究热点。目的:探讨组织工程化技术修复重建损伤半月板的可行性。方法:对体外构建组织工程化半月板的种子细胞进行培养,并制备半月板支架材料,将种子细胞依附于支架材料上,利用细胞因子调控种子细胞的黏附、生长、分化和迁移,组织学检查细胞与支架的结合情况以及细胞的数量等。结果与结论:组织工程化半月板修复研究主要包括种子细胞、支架材料和细胞因子等方面。构建需要的种子细胞有骨髓间充质干细胞和半月板纤维软骨细胞,半月板纤维软骨细胞传至第3代可得出最佳效应浓度。对组织工程化半月板支架材料进行表面修饰,由多材料组成的复合材料具有更好的生物相容性。应用组织工程化技术修复重建损伤的半月板。

BACKGROUND： The effect of autologous or al ogeneic transplantation in the repair of damaged meniscus is not ideal, so the application of tissue engineering technology in meniscus reconstruction has become the research focus. OBJECTIVE： To investigate the feasibility of tissue engineering technology for repair and reconstruct damaged meniscus. METHODS： The seed cells used for in vitro construction of tissue engineering meniscus were cultured, and the meniscal scaffold materials were prepared, then the seed cells were attached on the scaffold materials, and the cytokine was used to regulate the adhesion, growth, differentiation and migration of seed cells. Histological examination was performed to observe the binding between cells and scaffolds, as wel as the number of the cells. RESULTS AND CONCLUSION： The research of tissue-engineered meniscal repair included seed cells, scaffold materials and cytokines. The seed cells were constructed, including bone marrow mesenchymal stem cells and meniscal fibrocartilage cells, and the best effect concentration could be obtained after the meniscal fibrocartilage cells passaged to the third generation. Surface modification of tissue-engineered meniscus scaffold materials was performed, and the multi-material composed composite materials have better biocompatibility. The repair and construction of damaged meniscus with tissue engineering technology is the new treatment mode for repair of meniscus damage.