亚硒酸钠对大鼠胃癌变前期胃窦粘膜G、D和EC细胞的影响

EFFECTS OF SODIUM SELENITE ON G,D AND EC CELL IN ANTRAL MUCOSA OF EXPERIMENTALLY INDUCED PRECANCEROUS LESIONS IN RATS

目的 :探讨亚硒酸钠对大鼠胃癌变前期胃窦粘膜 G细胞、D细胞和 EC细胞的影响。方法 :用免疫组织化学及图像分析法 ,观察亚硒酸钠对 N-甲基 - N’-硝基 - N-亚硝基胍诱发的大鼠胃癌变前期胃窦粘膜 G细胞、D细胞和 EC细胞的变化。结果 :N-甲基 - N’-硝基 - N-亚硝基胍短期灌胃后 (阳性对照组 ) ,胃窦粘膜 G、D、EC细胞的数量和平均光密度值 (OD值 )与正常对照组比较均显著减少 (P<0 .0 1)。 N-甲基 - N’-硝基 - N-亚硝基胍灌胃后给含亚硒酸钠的硒饲料 ,低剂量硒组 G细胞数量与阳性对照组比较无明显变化 (P>0 .0 5) ,但 OD值明显增高 (P<0 .0 1) ;高剂量硒组 G细胞数量及 OD值均显著高于阳性对照组 (P<0 .0 5,P<0 .0 1) ;低硒组和高硒组 EC细胞数及 OD值与阳性对照组比较 ,均有显著性差异 (P<0 .0 5,P<0 .0 1) ;D细胞与阳性对照组比较无变化。结论 :胃窦粘膜 G细胞。

To study the influence of sodium selenite on G, D and EC cell in antral mucosa of experimentally precancerous lesions. Methods: Using immunohistochemistry ABC methods and image analysis, the effects of sodium selenite on G, D and EC cell in antral mucosa of precancerous lesions by N methyl N' nitro N nitrosoguanidine(MNNG) were oserved. Results: the numbers and optic density(OD) of G, D and EC cells in antral mucosa were significantly decreased after given MNNG for a short term (P<0.01). The forage contained sodium selenite was applied after given MNNG in rats. The number of G cell in low dose group were not changed compared with control group (P>0.05), but the optic density was significantl increased (P<0.01). The numers and optic density of G cell in high dose group were significantly increased compared with control (P<0.05, 0.01 respectively). The numbers and optic density of EC cell in low and high dose groups were significantly increased compared with control group(P<0.05, 0.01 respectively). No significant change was found in D cell. Conclusion: G cell, EC cell in antral mucosa are possibly involved in anti precancereous lesions of sodium selenite in rats.