摘要
目的 :野葛毛状根的诱导和无激素离体培养。方法 :用含超强毒性基因的发根农杆菌R16 0 1与野葛离体叶片共培养。结果 :经感染的野葛叶片表面直接形成大量生长快、分枝多、负向地性的毛状根 ,毛状根离体培养具有抗卡那霉素特性和激素自给特性。结论 :建立了发根农杆菌诱导野葛毛状根的方法和野葛毛状根的离体培养系统。
Objective: Tostua the hairy root induction and hormone-free in vitro liquid cultivation of Pueraria lobata (Willd) Ohwi. Method: Co-cultivation of super-virulent Agrobacterium rhizogenes R1601 with P. lobata leaves in uitro. Results: Hairy roots of rapid growth, high branches and plagiotropism developed vigorously on the surface of leaves, exhibiting rapid growth and resistance to kanamycin in hor-mone-free medium in vitro. Conclusion: A method of hairy root induction with A. rhizogenes as well as a system hairy root liquid cultivation in uitro for P.lobata have been established. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2000年第9期525-527,535,共4页
China Journal of Chinese Materia Medica
基金
广东省重点科技项目!资助 (S980 35 1)
江西自然科学基金!(S9940 1)
关键词
野葛毛状根
离体培养
诱导
Pueraria lobata
hairy root
cultivation in vitro
