EGFR和K-ras在结直肠癌中的表达及其临床意义

Expression and clinical significance of EGFR and K-ras in colorectal cancer

【目的】检测结直肠癌中表皮生长因子受体(epidermal growth factor receptor,EGFR)和K-ras蛋白的表达情况,分析其在结直肠癌组织中表达与临床病理特征之间的关系。【方法】应用免疫组化SP法检测80例原发性结直肠癌及其相应的癌旁组织中EGFR和K-ras蛋白的表达情况。【结果】EGFR、K-ras蛋白在结直肠癌组织中的阳性表达显著高于其在癌旁组织中的表达(P<0.01)。EGFR蛋白的表达与肿瘤病理分化程度、浸润深度以及肿瘤Dukes分期、有无淋巴转移有关,Dukes分期越晚(χ2=8.935,P=0.030),分化程度越低(χ2=11.757,P=0.001),浸润深度越深(χ2=6.888,P=0.009)及有淋巴结转移者(χ2=10.889,P=0.001),其表达越高,而与患者年龄、性别、肿瘤大小、肿瘤部位无关(P>0.05);K-ras蛋白仅与肿瘤的病理分化程度有关,分化程度越低(χ2=9.528,P=0.001),其阳性表达越多。结直肠癌组织中EGFR和K-ras蛋白的共表达呈正相关性(r=0.327,P=0.002)。【结论】EGFR和K-ras在结直肠癌中存在共表达现象,二者的高表达可能促进结直肠癌的发生、发展,检测二者可辅助判断患者的预后和转移情况以及指导临床治疗。

【Objective】To analysis the expression of epidermal growth factor receptor（EGFR） and K-ras protein in colorectal cancer and its possible relationship with its expression with the clinicopathological characteristics of colorectal cancer.【Methods】The expression of EGFR and K-ras protein were examined in 80 patients with primary colorectal cancer and adjacent normal tissues by immunohistochemical SP technique.【Results】The positive expression of EGFR,K-ras protein in colorectal cancer tissues was significantly higher than its expression in adjacent normal tissues（P 0.01）.EGFR protein expression of do related to the tumor histological differentiation,depth of invasion,Dukes stage tumors and lymph node metastasis.When the Dukes stage later（χ2 = 8.935,P = 0.030）,the lower the degree of differentiation（χ2= 11.757,P = 0.001）,the deeper the depth of invasion（χ2= 6.888,P = 0.009） and lymph node metastasis（χ2= 10.889,P = 0.001）,the higher expression of EGFR protein,but has nothing to do with the patient＇s age,gender,tumor size,tumor site（P 0.05）.K-ras protein is only related to pathological differentiation of the tumor,the lower the degree of differentiation,the more positive expression.EGFR and K-ras protein expression was positively correlated with colorectal cancer tissues（χ2= 9.528,P = 0.001）.【Conclusion】EGFR and K-ras co-express in colorectal cancer.Their high expression may contribute to the occurrence and development of colorectal cancer.Testing them may help to determine the prognosis and metastasis,guide clinical treatment.