溃疡散对大鼠胃溃疡的保护作用

Protective effect of Kuiyangsan on gastric ulcer in rats

【目的】通过建立幽门结扎型大鼠胃溃疡模型,研究溃疡散对胃溃疡的防治作用。【方法】随机将50只Wista雄性大鼠分成5组,每组10只,即:阴性对照组,溃疡散低、中、高剂量组,阳性对照组。溃疡散高、中、低3个剂量组分别灌胃溃疡散0.52g/kg,0.26 g/kg,0.13g/kg,3次/d。阴性对照组:制模方法同上,生理盐水灌胃,2ml/次,3次/d。阳性对照组:雷尼替丁灌胃0.27g/kg,3次/d。各组连续给药5 d,第5日灌胃后禁食24 h。于第6日,乙醚麻醉后制备幽门结扎型大鼠胃溃疡模型。计算胃液总酸度,胃蛋白酶活性,溃疡指数。【结果】溃疡散高、中、低剂量组溃疡指数分别为1.52±0.41、2.13±0.56、2.76±0.75与阴性对照组5.01±0.82相比,有显著性差异(P<0.05)。胃液量分别为7.19±0.14、8.51±0.43、9.34±0.61ml均高于阴性对照组5.01±0.82ml(P<0.05)。总酸度分别为86.08±7.19、90.94±19.21、96.17±13.71 mEq/L,低于阴性对照组126.8±17.5 mEq/L(P<0.05)。胃蛋白酶活性分别为151.43±10.37、165.41±16.36、190.32±8.36 u/ml,与阴性对照组290.55±14.21 u/ml有显著性差异(P<0.05)。【结论】溃疡散能够有效减少胃酸分泌量、胃蛋白酶活性,防止胃溃疡的发生。

【Objective】To determine the effect of Kuiyangsan on gastric ulcer（GU）.【Method】Gastric ulcer in rat was established by ligating the pylorus of the rat.Fifty healthy male Wista rats were equally devided into five groups in random.High dose kuiyangsan group,middle dose kuiyangsan group,low dose kuiyangsan group were intragastrically fed with kuiyangsan（0.52,0.26,0.13 g/kg）.Positive control group was intragastric fed with ranitidine 0.27 g/kg.Negative control group was intragastric fed with water.Intragastric fed was given every group for five days,at the fifth day,the rats were fasted for 24 h.At the seventh day of the pylorus of fifty GC rats were ligated after anaesth.Totle gastric acid,pepsin activity,ulcer exponent of rats were measured.【Results】The exponent of uler in high dose kuiyangsan group,middle dose kuiyangsan group,low dose kuiyangsan group and negative group.Ulcer index were 1.52± 0.41,2.13±0.56,2.76±0.75 and 5.01±0.82 respectively.Kuiyangsan group tended to be lower than negative group（P 0.05）.Gastric fluid volum of high dose kuiyangsan group,middle dose kuiyangsan group,low dose kuiyangsan group and negative group were 7.19± 0.14,8.51±0.43,9.34±0.61,501±0.82ml.Kuiyangsan group tended to be lower than negative group（P 0.05）.Totle gastric acid of high dose kuiyangsan group,middle dose kuiyangsan group,low dose kuiyangsan group and negative group’s were 86.08±7.19,90.94±19.2,96.17±13.7 and 126.8±17.5 mEq/L respectively.Kuiyangsan group tended to be lower than negative group（P 0.05）.Peptic activity of high dose kuiyangsan group,middle dose kuiyangsan group,low dose kuiyangsan group and negative group’s were 151.43±10.37,165.41±16.36,190.32±8.36 and 290.55±14.21 u/ml respectively.Kuiyangsan group tended to be lower than negative group（P 0.05）.【Conclusion】Kuiyangsan could reduce gastric acid volum,inhibit pepsin activity and prevent the occurrence of gastric ulcer.