C-MYC基因与老年胃癌及直肠癌的关系研究

C-MYC oncogene amplification in elderly stomach and colon carcinomas patients

目的 研究老年人胃癌和直肠癌中的 C- MYC基因扩增情况。方法 用聚合酶链式反应 (PCR)测定 84例老年胃癌和直肠癌患者中的 C- MYC基因 ,通过激光密度扫描仪对琼脂糖凝胶 EB染色底片进行积分光密度分析。结果 正常组织无 C- MYC扩增 ,C-MYC/N+L- MYC比值 95%可信区间为 0 .0 86 9～ 0 .62 5 7,胃癌组织 C- MYC基因扩增阳性率为 69.2 % ,直肠癌组织的 C- MYC基因扩增阳性率为 62 .5% ,84例肿瘤组织 C- MYC扩增总阳性率为 66.7%。结论 癌组织 C- MYC扩增高于正常组织 ,在不同肿瘤组织中扩增程度不同 ,与细胞潜在的增殖能力成正相关 ;这可为肿瘤的发生、发展及预后 ,特别为指导基因治疗提供有用的资料。

To study the amplification of c myc gene in 84 case with different tumors.Methods c myc gene was detected by means of polymerize chain reaction(PCR), and the results in films staining with EB were analyzed by integral density.Results the amplification of c myc gene was not found in 32 normal controls, and the average density of c myc/n+l myc was 0 086 9～0 625 7. The positive rate of c myc amplification in carcinoma of colon was 62 5%, the positive rate of c myc amplification in 52 stomach cancers was 69 25%, the positive rate was 66 7% in the total 84 differet cancers cases.Conclusions c myc gene was related with cell growth, differentiation and tumorous prognosis, the detecting c myc amplification was useful for gene therapy with antisense oligonucleotide.