鼻咽癌组织HSP27与P53、P21癌基因蛋白表达相关性研究

Study on the Expression of Heat Stress Protein 27, P53 and P21 in Nasopharyngeal Carcinoma

探讨鼻咽癌 (nasopharyngeal carcinoma,NPC)组织中热应激蛋白 2 7(heat stress protein 2 7,HSP2 7) ,P5 3及 P2 1癌基因蛋白表达水平及其相互关系 ,采用间接免疫荧光染色和 SABC相结合的双重荧光染色法 ,分析 3种蛋白在鼻咽癌发生、发展及预后中的作用。结果发现 :32例 NPC中 HSP2 7,P5 3和 P2 1阳性表达比例 (阳性率 )分别为83.8%、 5 0 %、 93.8% ;8例鼻咽增生性炎症组织中三者比例为 4/ 8、 0 / 8、 4/ 8;4例正常组织则全部阴性表达。 HSP2 7、P5 3、 P2 1的共表达以 HSP2 7与 P2 1的共表达最为显著 ,且与 NPC恶性程度和临床分期有关。提示 :HSP2 7及 P2 1阳性表达可能是 NPC发生的早期变化 ,可将二者联合作为 NPC早期诊断及预防的敏感生物标志物。

The expression of heat stress protein 27 (HSP27), P53 and P21 in nasopharyngeal carcinoma (NPC) and the role of the three proteins in the occurrence, development and prognosis of NPC were studied. Indirect immunofluorescence method combined with SABC method was used to detect the expression of HSP27, P53 and P21.The results showed HSP27, P53 and P21 were expressed in 83 8 %, 50 % and 93 8 % of all NPC patients and in 4/8,0/8,4/8 of hyperplastic nasopharyngitis, respectively. In normal tissues of 4 cases, all were negatively stained. Coexpression of HSP27 and P21 was the most obvious among the coexpression of HSP27, P53, P21, and it was related with the malignant degree and the clinical stage of NPC. It was concluded that HSP27 and P21 expression might be an earlier event in NPC, and both of them could be regarded as the biomarkers of diagnosis and prevention of NPC at early stage.