汉防己甲素抗肝纤维化的形态计量学研究

Morphological study on the anti hepatic fibrosis of tetrandrine in rats

[目的 ]观察汉防己甲素的抗肝纤维化的作用 .[方法 ]将 72只大鼠随机分组 .用 5 0 %四氯化碳油剂皮下注射 14周制作大鼠肝纤维化模型 .治疗组用汉防己甲素灌胃 ,连续 14周 ,第 4,8,14周取肝组织作切片 ,着重观察肝细胞脂肪变性、纤维化程度 ,并利用彩色图象分析系统测量胶元纤维的面积 .[结果 ]中毒组肝细胞脂肪变性从第 4周开始逐渐加重 ,第 14周有所减轻 ,肝纤维化从第 4周开始逐渐加重 ,第 14周形成假小叶 ;治疗组肝细胞脂肪变性、肝纤维化程度比中毒组轻 ,肝组织内胶原含量明显减少 ,与中毒组比较有显著性差异 .汉防己甲素的疗效每kg体重 5 0mg组优于 30mg组 .[结论

OBJECTIVE To investigate the effects of tetrandrine on hepatic fibrosis in rats. METHODS The rats were randomly divided into some groups.Liver fibrosis was induced in rats by subcutaneous injection of 50% carbone tetrachloride oil solution for a period of 14 weeks. The treatment groups were treated with tetrandrine twice a week for 14 weeks. Histopathological examination was made in 4?th ,8?th,14?th week after experiment.A quantity of collagenous fibers were determined by using color image analysis system. RESULTS Hepatic steatosis gradually increased from 4?th week and decrease in 14?th week. Hepatic fibrosis gradually increase from 4th week and pseudo lobules are formed in 14th week. Hepatic steatosis and fibrosis in treatment groups are slighter than those in toxic group.A quantity of collagenous fibers in liver tissue are significantly lower in treatment groups than in toxic group. The effects of tetrandrine at 50?mg/kg dose are better than those at 30?mg/kg. CONCLUSION Tetrandrine has good effects in the treatment of hepatic steatosis and fibrosis induced by carbone tetrachloride oil in rats.