富硒麦芽对阿霉素致小鼠心脏毒性的保护作用

The protective action of Malt-Se to the cardiotoxicity of doxorubicin by Malt-Se

目的 :探讨含硒制剂诱导金属硫蛋白在对抗阿霉素心脏毒性中的作用 ,比较无机硒制剂亚硒酸钠与有机硒制剂富硒麦芽。方法 :不同时间给小鼠喂予硒制剂后取血分离血清 ,按常规查GOT和CK活性 ,按DTNB法测GSH Px活性 ;取心、肝、肾组织按Hg Chelex法测金属硫蛋白 (MT)含量并做心脏病理学检查。结果 :两种含硒制剂均能减轻阿霉素引起的体重下降、GOT与CK酶升高及心脏病理损害 ,其中富硒麦芽的效果略优于亚硒酸钠。两者还能增加GSH Px酶活性 ,诱导心脏中MT合成增加 ,连续使用硒制剂的整体效果优于先或后使用该制剂。结论 :除升高GSH Px外 ,硒制剂诱导合成MT很可能是减轻阿霉素心脏毒性的保护机制之一。两种硒制剂比较使用富硒麦芽较亚硒酸钠更为安全 ,且不降低原已证实的亚硒酸钠对心肌的保护作用。

OBJECTIVE:To study the effect of MT during Na 2SeO 3 and MaltSe on the cardictoxicity produced by doxorubicin,and to compare organic and inorganic compounds containingSe.METHODS:The measurement of GOT and CK in blood serum and examination of the pathological heart damage was done with routine methods.The GSHPx activity in blood serum was assayed by DTNB method.Hgchelex method was used for determination of MT in heart,liver and kidney.RESULTS:Both of the compounds can resist the toxicities of doxorubicin,such as the body weight decreased,GOT and CK increased and pathological heart damage(P<0.01 or P<0.05).They also increase the activity of GSHPx(PP<0.01) and induce metallothionein(MT) in heart,liver and kidney(P<0.01 or P<0.05).The effects of using the compounds continuously is better than that of preadministration or retroadministration.CONCLUSION:Those suggeste that GSHPx and MT may all have the cardicprotective effects,and organic compound(Malt-Se) may be safer than inorganic one(Na 2SeO 3).