毛细管气相色谱法测定人尿中右美沙芬及其代谢物

Determination of dextromethorphan and its metabolite in urine by capillary GC

目的 :为研究细胞色素P4 50同工酶CYP2D6在人群中的代谢多态性 ,本文建立了人尿中右美沙芬 (DM)及其O 去甲基代谢物 3 羟基 N 甲基 吗喃 (DT)的气相色谱分析方法。方法 :尿样经提取后 ,以HP 1毛细管柱作为分离柱 ,FID为检测器 ,正二十二烷为内标进行气相色谱分析。结果 :在此实验条件下 ,右美沙芬、内标物和代谢物的保留时间分别为 8.5,9.9和 1 1 .2min ;尿样中DM在 0 .1 0～ 2 .0 μg/ml、DT在 0 .2 0～ 2 0 .0 0 μg/ml范围内线性关系良好 ,回收率分别 95.7%～ 1 0 4 .4 % (DM )和 93 .3 %～ 98.3 % (DT) ;样品测定的RSD分别为 2 .5%和 3 .1 %。结论 :该法简便可靠 ,能满足CYP2D6代谢多态性研究的要求。

OBJECTIVE:To establish a capillary gas chromatography method for the determination of dextromethorphan(DM) and its metabolite Dextrorphanol(DT) to study the metabolic polymorphism of CYP2D6 in Chinese.METHODS:Urine samples were extracted,and analysed on HP 1 capillary column.Docosane was chosen as the internal standard(IS).The detector used was FID.RESULTS:Under the optimal GC conditions,the retention times of DM,IS and DT were 8.5,9.9 and 11.2min,respectively.The assay was linear at the range of 0.1～1.60μg/ml(DM) and 0.20～20.000μg/ml(DT).The absolute recoveries from urine were 95.7%～104.4%(DM) and 93.3%～98.3%(DT),respectively.The RSD of the assay was 2.54% for DM and 3.14% for DT.CONCLUSION:The results showed that the GC method developed was simple,rapid and reliable,and was suitable for study of the metabolic polymorphism of CYP2D6.