二阶导数分光光度法测定复方美沙芬布洛芬含量

Determination of dextrometomethorphan hydrobromide and ibuprofen in compoud capsules by the second derivative spectrophotometry

目的 :测定复方美沙芬布洛芬含量。方法 :以甲醇为溶剂 ,采用二阶导数分光光度法 ,用峰 谷法测定 ,美沙芬、布洛芬的测定波长分别为 2 92 .3nm、2 88.2nm和 2 60 .8nm、2 63 .8nm。结果 :美沙芬在 3 .0 7～ 1 5.3 5μg/ml,布洛芬在 3 9.88～ 1 99.4 0 μg/ml范围内呈线性关系 ,其平均回收率分别为 98.2 %和 98.7% ,RSD皆为 0 .4 %～ 0 .7%。 结论 :本法快速、准确 ,适合于该制剂的含量测定。

OBJECTIVE:To determine the contents of dextromethorphan hydrobromide and ibuprofen in compoud capsule.METHODS:A second derivative spectrophotometric method was used by peak valley method for the determination of dextromethorphan hydrobromide and ibuprofen in the solvent of methanol.Their detection wavelengths were 292.3nm,288.2nm and 260.8nm,263.8nm,respectively.RESULTS:The linear ranges of dextromethorphan hydrobromide and ibuprofen were 3.07～15.35μg/ml,39.88～199.40μg/ml,and their mean recoveries were 98.2% and 98.7% with RSD in the range of 0.4%～0.7%.CONCLUSION:The method was rapid,accurate,and suitable for the analysis of this preparation.