X射线全身照射对小鼠腹腔巨噬细胞NO产生的影响

Effect of whole body X irradiation on production of nitric oxide in peritoneal macrophages of mice\

目的 观察 75mGy、2 0GyX射线全身照射后不同时间 ,小鼠腹腔巨噬细胞NO产生的时程变化及不同剂量照射后 2 4h时NO、iNOS的含量变化。方法 分光光度法及免疫组织化学方法。结果 iNOS和NO的产生与剂量呈正相关。 2 0Gy照射后 ,NO从 6h到 48h随时间推移产生量逐渐增加 ,48h达峰值 ,为对照的 3 8倍 ,而后回降。

Objective\ To disclose the changes of nitric oxide (NO) in murine peritoneal macrophages at different time intervals after whole body irradiation (WBI) with 75 mGy and 2\^0 Gy X\|rays and the changes of NO and induced nitric oxide synthase (iNOS) 24 hours after WBI with 0\^05-6\^0 Gy X\|rays. Methods\ Immunohistochemistry and spectophotometry were used. Results\ The expression of iNOS and the production of NO increased in a dose\|dependent manner after irradiation.The production of NO increased from 6h to 48h and reached its peak at 48h,which was 3\^8\|fold of sham\|irradiated controls. Conclusion\ Ionizing radiation stimulates expression of iNOS and production of NO in peritoneal macrophages of mice.\;