肿瘤坏死因子α对小鼠骨髓型放射损伤的防护作用

Radioprotective effects of tumor necrosis factor-α on murine bone marrow damage

目的 研究肿瘤坏死因子α(TNFα)对受照射小鼠骨髓造血细胞的近期影响和造血重建功能的作用 ,探讨TNFα对骨髓造血细胞的辐射防护作用。方法 小鼠照射前 2 4h一次性注射TNFα,2周后检测其外周血WBC、骨髓MNC、CFU -S和CFU -Mix ,流式细胞仪分析骨髓c Kit+细胞 ;并进行长期造血重建实验。结果 辐照前注射TNFα的小鼠 ,2周后外周血WBC、骨髓MNC、内源性CFU -S、CUF -Mix数量和c Kit+细胞百分数均显著高于未注射TNFα的辐照小鼠 ,生存期亦延长 ;接受TNFα处理的小鼠骨髓细胞移植到受致死性照射的受体鼠后 ,多具有良好的造血重建功能。

Objective To investigate the effects of tumor necrosis factor α(TNFα)on the bone marrow (BM)hematopoietic progenitor cells (HPCs)in irradiated mice and the radioprotection of TNFα on BM HPCs. Methods TNFα was injected into the mice before irradiation. Peripheral WBC, BM mononuclear cells (MNCs), CFU-S, CFU-Mix and hematopoietic reconstitution ability were examined on lethally irradiated mice with or without TNFα pre\|treatment. Surface marker and c\|Kit + of BM stem cells was analyzed with flow cytometry. Results The mice with TNF α treatment were markedly higher than those without TNFα treatment in number of WBC, BM MNCs, CFU-S, and CFU-Mix in vitro and in the percentage of BM c\|Kit + cells. The life span of the former was longer than that of the latter. In hematopoietic reconstitution, the above criteria of the recipient mice given the BM cells derived from donor mice of pre\|irradiation TNFα treatment were remarkable superior to those given the donor without TNFα treatment. Conclusion Pre irradiation treatment has obviously radioprotective effect on the BM HPCs. The experiment provides a novel approach to lessening BM hematopoietic arrest caused by chemothrapy and radiotherapy in tumor patients, and is of great significance in clinical application.