内皮型一氧化氮合酶在急性放射性脑水肿中的表达

Expression of endothelial nitric oxide synthase in acute radiation induced cerebral edema

目的 探讨大剂量60 Coγ刀照射后脑血管内皮细胞内皮型一氧化氮合酶 (eNOS)表达变化及其与急性放射性脑水肿的关系。方法 单次 2 0 0Gy60 Coγ刀照射大鼠右侧尾状核头部 ,于照射后 14d内分批活杀动物 ,采用光镜、电镜、免疫组化及原位杂交技术研究照射后急性放射性脑水肿的发生发展及脑血管内皮细胞eNOS表达变化。结果 照射后 2h出现急性放射性脑水肿病理改变 ,照射后 3d达高峰 ;脑血管内皮细胞eNOS于照射后 2h亦出现明显表达增高 ,且持续时间长 ,伴行于放射性脑水肿急性发展阶段。结论 大剂量60

Objective To study the relation between changes in expression of endothelial nitric oxide synthase(eNOS)in brain vascular endothelial cells and acute radiation\|induced cerebral edema after 60 Co gamma knife irradiation with a high dose. Methods The right caudate nucleus of rats were irradiated with 200 Gy 60 Co gamma knife. The rats were sacrificed within 14 days after irradiation. By a light microscopy, electron microscopy, immunohistochemistry and in situ hybridization, we studied the formation and development of acute radiation induced cerebral edema as well as the expression of eNOS in brain vascular endothelial cells. Results Acute radiation induced cerebral edema was observed morphologically 2 hours after irradiation and peaked on day 3 after irradiation. The expression of eNOS in brain vascular endothelial cells markedly increased 2 hours after irradiation, which could be consistently visualized during the acute developmental phase of radiation induced cerebral edema. Conclusion The increased expression of eNOS may be related to the formation and development of acute radiation induced cerebral edema after 60 Co gamma knife irradiation with a high dose.