新藤黄酸纳米脂质载体制备及其药剂学性质研究

Preparation of gambogenic acid loaded nanostructured lipid carriers and its pharmaceutical properties

目的制备新藤黄酸纳米结构脂质载体并表征其药剂学性质。方法采用乳化蒸发-低温固化法制备新藤黄酸纳米脂质载体(GNA-NLC),正交试验设计优化最佳工艺处方,并对其包封率、平均粒径及Zeta电位等性质进行考察。结果优化后处方制备的GNA-NLC多为圆整、实体的类球形,平均粒径为(144.07±1.44)nm,多分散系数为0.24±0.01,Zeta电位为(28.03±0.29)mV,包封率为(84.65±0.98)%,载药量为(4.21±0.05)%;DSC显示GNA纳米粒确已形成,并且GNA以非晶态分布在基质中。结论乳化蒸发-低温固化法能成功制备GNA-NLC,工艺简单,易于控制。

Objective To prepare and characterize gambogenic acid loaded nanostuctured lipid carrier （GNA-NLC）. Methods GNA-NLC was prepared by emulsion evaporation-low temperature solidification method, and its optimal prescription was selected by orthogonal test design. The characteristics, such as encapsulation efficiency （EE）, average diameter, and Zeta potential, were studied. Results GNA-NLC had a spherical shape with smooth surface with the average diameter of （144.07 ± 1.44） nm, polydispersity index （PI） of 0.24± 0.01, Zeta potential of （-28.03± 0.29）mV. The EE and drug loading were （84.65v 0.98）% and （4.21± 0.05）%, respectively. DSC indicated that GNA was dispersed as non-crystalline in matrix. Conclusion Emulsion evaporation-low temperature solidification method is easy, simple, and feasible to prepare GNA-NLC.