人喉鳞癌细胞67-KD层粘连蛋白受体的表达

Expression of 67-KD LN-R in Human Laryngeal Squamous Carcinoma Cells

目的观察 6 7-KD层粘连蛋白受体 (6 7-KDlamininreceptor,6 7-KDLN -R)在人喉鳞癌细胞中的表达情况 ,并分析其表达与临床病理特征间的关系。 方法采用免疫组化SP方法检测 30例喉鳞癌原发灶和其中的 3例颈淋巴结转移灶石蜡标本中 6 7-KDLN -R的表达。 结果 6 7-KDLN -R在喉鳞癌原发灶中的阳性率为5 6 .7% (1 7/ 30 )。转移淋巴结的免疫反应结果与原发部位的免疫反应结果相一致。 6 7-KDLN -R的表达与肿瘤的分化程度密切相关 ,分化差者其表达水平更高 (P <0 .0 5 )。与无颈淋巴结转移的喉鳞癌细胞相比 ,有颈淋巴结转移喉鳞癌细胞 6 7-KDLN -R的表达水平更高 (P <0 .0 5 )。 结论 6 7-KDLN -R在喉鳞癌分化、增殖、浸润及转移中起重要作用。

Objective To observe the expression of 67-KD laminin receptor (67-KD LN-R) in human laryngeal squamous carcinoma cells and to analyse the correlation between its expression and clinicopathological characters. Methods We examined the expression of 67-KD LN-R in 30 primary laryngeal squamous carcinomas and in 3 cervical metastatic lymph nodes by the immunohistochemical SP method on paraffin-embedded sections. Results The positive rate of 67-KD LN-R in the primary site of laryngeal squamous carcinomas was 56.7% (17/30). The immunoreactivity in 3 cervical metastatic lymph nodes was consistent with that in primary tumors. There was a significant correlation between the level of 67-KD LN-R and the degree of tumor differentiation, the level being higher in poorly differentiated tumors (P<0.05). The level of 67-KD LN-R in laryngeal squamous carcinomas with cervical lymph node metastases was higher than that in those without cervical lymph node metastases (P<0.05). Conclusion 67-KD LN-R plays an important role in the differentiation, proliferation, invasiveness and metastasis of laryngeal squamous carcinoma.