支气管活检标本的端粒酶活性检测对肺癌的诊断价值

The diagnostic value of telomerase activity in bronchial biopsy specimen for lung cancer

目的 探讨肺癌组织端粒酶活性检测对肺癌的诊断价值。方法 采用端粒酶重复序列扩增法检测纤维支气管镜活组织检查的肺癌病变组织 (70例 )、肺癌对侧支气管组织 (70例 )及非癌性肺疾病支气管组织 (2 0例 )的端粒酶活性 ,并和病理学及细胞学检查结果进行比较。结果 肺癌病变组织端粒酶检出率为 81% ,明显高于肺癌对侧支气管组织 (14% ) (P <0 0 1)及非癌性肺疾病支气管组织 (10 % )的检出率 (P <0 0 1) ;肺癌组织端粒酶检出率与活组织检查阳性率 (87% )及刷检阳性率(76 % )比较差异均无显著性 (P >0 0 5 )。结论 端粒酶活性的检测 ,对肺癌的诊断有重要意义。

Objective To explore the diagnostic value of telomerase activity for lung cancer Methods Telomerase activity was measured by PCR TRAP in cancerous lesions and contralateral bronchial tissues from patients with lung cancer and in the bronchial tissues from patients with noncancerous pulmonary disorders,in which the tissue specimens were obtained by fiberoptic bronchoscopy.The result of telomerase detection was compared with the positive rates of pathologic study and bronchial brush cytology Results The positive rate of telomerase activity in lesions of lung cancer was significantly higher than that in tissues of the contralateral bronchi from patients of lung cancer ( P <0 01) and that in tissues from patients of noncancerous pulmonary disorders ( P <0 01) There was no significant difference between the positive rate of telomerase activity expression and the positive rates of pathology and bronchial brush cytology ( P >0 05) Conclusion Examination of telomerase activity was of significance in the diagnosis of lung cancer