生长抑素对奥迪括约肌运动的作用机制

The regulatory mechanism of somatostatin on sphincter of Oddi motility

目的 了解生长抑素影响奥迪括约肌运动的神经通路成分。方法 建立奥迪括约肌测压慢性实验动物模型 ,研究 M受体阻断剂、α受体阻断剂、β受体阻断剂、5- HT4 受体阻断剂、一氧化氮合酶抑制剂和河豚毒素对生长抑素调节奥迪括约肌运动的影响。结果 阿托品、二乙胺盐酸甲酯和河豚毒素阻断生长抑素对括约肌的兴奋作用 ;L -精氨酸甲酯阻断生长抑素对括约肌的抑制作用。结论 生长抑素通过胆碱能和 5-羟色胺能神经元组成的兴奋性神经通路 ,兴奋奥迪括约肌 ;通过含一氧化氮神经元的非肾上腺素能非胆碱能神经通路 ,抑制奥迪括约肌。

Objective To clarify the composition in the nervous pathway via which somatostatin regulates the sphincter of Oddi motility.Methods Dogs were selected to establish the chro nic manometry model to record the sphincter of Oddi motility.Effects of somatostatin on sphincter of Oddi motility were studied by the injection of M receptor blocker,α receptor blocker,β receptor blocker,5 HT 4 receptor blocker,nitric oxide synthase inhibitor and tetrodotoxin.Results Atro pine,diethylamine methyl ester hydrochloride and tetrodotoxin could abolish the excitatory role of somatostatin on the sphincter.N nitro L arginine methyl ester could reverse the inhibitory role of somatostatin on the sphincter.Conclusions Somatostatin stimulates sphincter of Oddi motility via an excitatory nervous pathway consisting of cholinergic and 5 hydroxytryptaminergic neurons;it inhibits the sphincter via a nonadrenergic and noncholinergic inhibitory nervous pathway containing nitric oxide neurons.