多发性骨髓瘤患者中N-ras基因突变的检测及其意义

Detection of N-ras Gene Mutations in Multiple Myeloma and Its Si gnificance

目的 了解多发性骨髓瘤 (multiplemyeloma ,MM)患者中N ras基因突变率及临床意义。方法 采用聚合酶链反应 单链构象多态性银染法检测MM患者的N ras基因突变 ,并结合临床分析。结果 14例MM患者中 ,4例在N ras第 6 1密码子 ,1例在第 12、13密码子处发生突变 ,突变率为 35 .7%。突变者中 4例已死亡 ,其中 2例检测突变时已处于疾病终末期。结论 N ras基因在MM中有较高的突变率 ,对其的检测有助于病程进展和预后的分析。

Objective To investigate mutation rate of N-ras in multiple myeloma(MM) and discuss the clinical significance of MM associated with N-ras gene mutations.Methods The N-ras gene mutations in 14 cases of MM were detected by the methods of polymeras chain reaction-single strand conformation polymorphism (PCR-SSCP) and silver staining technique. Both clinical features and laboratory results were analyzed simultaneously.Results There were mutation of N-ras in five cases (35.7%), four in codon 61, and one in codon 12 or 13. Four of them died, and two of them were detected mutations at the terminal stage of the disease.Conclusion It showed that a relative high frequence of N-ras gene mu tation was found in MM. And detecting N-ras gene mutations in MM might be benef it to evaluate the progression and prognosis of disease.