小鼠结肠腺癌肝转移模型的建立

Development of A Colonic Adenocarcinoma Liver Metastases Model in Mice

目的 建立结肠癌肝转移的动物模型 ,用于肿瘤转移防治的实验研究。方法 对BALB/c小鼠 ,经脾脏注入指数生长期的小鼠结肠腺癌细胞 (CT2 6 )悬液 0 .1ml,含细胞 1× 10 6个 ,保留脾脏。观察接种后小鼠的生存期 ,小鼠分别于接种后第 7、10、15天及自然死亡后剖腹 ,观察腹腔内肿瘤生长情况 ,留标本作病理检查和流式细胞术 (FCM )倍体检测。结果 小鼠平均自然生存时间为 (18.2± 1.8)天 ,尸解发现接种动物的肝脏表面均有灰白色转移结节形成 ,脾脏上有结节形成 ,其他脏器未见转移病灶。病理结果示肝转移瘤细胞与脾脏肿瘤细胞结构相似 ,符合低分化腺癌的特征。FCM倍体检测结果示肝转移瘤为四倍体肿瘤 ,与CT 2 6细胞倍体检测结果一致。结论 经脾脏注入CT 2 6细胞是建立结肠癌肝转移的可靠模型 ,具有潜伏期短、可重复的高转移发生率的特点 ,有较好的应用价值。

Objective To develop a colonic adenocarcinoma liver metastases model for experimental studies in mice.Methods Liver metastases were established in BALB/c mice by intrasplenic injection of colonic adenocarcinoma cells(CT26) with 1×10 6 cells in 0.1 ml solution. The spleen was left intact.The survival time of the animals was recorded.Animals were evaluated at the 7,10,15 day postinjection by abdominal autopsy,respectively.The liver metastases were studied histologically and ploid of tumor cells was determined by flow cytometry(FCM).Results The average survival time of animals was(18.2±1.8) days.All animals inoculated with CT26 cell lines developed liver metastases.Other organic metastases were not observed. The pathological results showed that tumor cells of liver metastases accorded with poorly differentiated colonic adenocarcinoma.FCM indicated that tumors of liver metastases were tetraploid,identical with that of CT26 cell lines.Conclusion Intrasplenic injection of CT26 cells is a reliable model for producing colonic cancer liver metastases and should prove valuable in experimental study.